Development of a facile electrochemical sensor based on GCE modified with one-step prepared PNMA-CeO2-fMWCNTs composite for simultaneous detection of UA and 5-FU

In this study, a poly(N-methyl aniline)-cerium oxide-functionalized MWCNTs (PNMA-CeO2-fMWCNTs) composite was synthesized in a one-step preparation technique. As a highly efficient modifier, the composite was used to modify the glassy carbon electrode surface for simultaneous detection of uric acid (UA) and 5-fluorouracil (5-FU). Morphological characterization of the GCE/PNMA-CeO2-fMWCNTs was studied using scanning electron microscopy. Structural characterization of the composite was performed using X-ray diffraction and Fourier-transformed infrared spectroscopy. Electron transfer properties of the prepared electrodes were carried out with electrochemical impedance spectroscopy and cyclic voltammetry. The linear working range for UA and 5-FU was found to be 0.25-50 mu M and 0.5-750 mu M, respectively. The limit of detection values for UA and 5-FU were 0.04 mu M and 0.19 mu M, respectively. The effects of various interfering substances on the electrochemical response of UA and 5-FU were investigated. The GCE/PNMA-CeO2-fMWCNTs sensor has excellent stability, reproducibility, anti-interference ability, and reproducibility. To demonstrate the practical application of the sensing platform, fetal bovine serum was selected and tested in the spiked samples, and satisfactory results were obtained. The prepared composite proved to be a promising platform for simple, rapid, and simultaneous analysis of UA and 5-FU.

Automated summary

In this study, a poly(N-methyl aniline)-cerium oxide-functionalized MWCNTs composite was synthesized and used as a modifier for simultaneous detection of uric acid and 5-fluorouracil. The composite exhibited excellent sensing performance with a wide linear working range and low detection limits. The effect of interfering substances on the electrochemical response was investigated, confirming the stability and anti-interference ability of the sensing platform.