期刊
PHARMACEUTICS
卷 15, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/pharmaceutics15112586
关键词
metabolites; antifungal drugs; azole derivatives; screen-printed electrodes (SPE); phthalocyanine electrodes; mass spectrometry; UHPLC; HLM; principal component analysis (PCA)
Understanding the metabolism of pharmaceutical compounds is essential for their safety and efficacy. The application of electrochemistry in drug metabolism studies offers a promising approach for synthesizing and analyzing drug transformation products. This study investigated the hepatic metabolism of voriconazole and optimized the electrochemical parameters to simulate the biotransformation reactions. The findings highlight the potential of phthalocyanine electrodes as an efficient and cost-effective tool in drug biotransformation research.
Understanding the metabolism of pharmaceutical compounds is a fundamental prerequisite for ensuring their safety and efficacy in clinical use. However, conventional methods for monitoring drug metabolism often come with the drawbacks of being time-consuming and costly. In an ongoing quest for innovative approaches, the application of electrochemistry in metabolism studies has gained prominence as a promising approach for the synthesis and analysis of drug transformation products. In this study, we investigated the hepatic metabolism of voriconazole, an antifungal medication, by utilizing human liver microsomes (HLM) assay coupled with LC-MS. Based on the obtained results, the electrochemical parameters were optimized to simulate the biotransformation reactions. Among the various electrodes tested, the chemometric analysis revealed that the iron(II) phthalocyanine electrode was the most effective in catalyzing the formation of all hepatic voriconazole metabolites. These findings exemplify the potential of phthalocyanine electrodes as an efficient and cost-effective tool for simulating the intricate metabolic processes involved in drug biotransformation, offering new possibilities in the field of pharmaceutical research. Additionally, in silico analysis showed that two detected metabolites may exhibit significantly higher acute toxicity and mutagenic potential than the parent compound.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据