An ultrasensitive fluorescent biosensor for glucose detection is developed using a Fenton-like reaction triggered chemical redox-cycling signal amplification strategy. The strategy allows for self-sustaining chemical redox-cycling reaction and remarkable fluorescent enhancement, enabling the detection of glucose with high sensitivity.
An ultrasensitive fluorescent biosensor is reported for glucose detection based on a Fenton-like reaction triggered chemical redox-cycling signal amplification strategy. In this amplified strategy, Cu2+ oxidizes chemically o-phenylenediamine (OPD) to generate photosensitive 2,3-diaminophenazine (DAP) and Cu+/Cu-0. On the one hand, the generated Cu-0 catalyzes the oxidation of OPD. On the other hand, H2O2 reacts with Cu+ to produce hydroxyl radicals ((OH)-O-center dot) and Cu2+ through a Cu+-mediated Fenton-like reaction. The generated (OH)-O-center dot and recycled Cu2+ ions take turns oxidizing OPD to produce more photoactive DAP, triggering a self-sustaining chemical redox-cycling reaction and a remarkable fluorescent enhancement. It is worth mentioning that the cascade reaction did not stop until OPD molecules were completely consumed. Benefiting from H2O2-triggered chemical redox-cycling signal amplification, the strategy was exploited for the development of an ultrasensitive fluorescent biosensor for glucose determination. Glucose content monitoring was realized with a linear range from 1 nM to 1 mu M and a limit of detection of 0.3 nM. This study validates the practicability of the chemical redox-cycling signal amplification on the fluorescent bioanalysis of glucose in human serum samples. It is expected that the method offers new opportunities to develop ultrasensitive fluorescent analysis strategy.
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