A new α-amylase inhibitory peptide from Gynura medica extract

Article Biochemistry & Molecular Biology

Design, synthesis, and molecular modeling of heterodimer and inhibitors of α-amylase as hypoglycemic agents

Ved Prakash Singh et al.

Summary: A series of rosiglitazone-based compounds were designed and synthesized, and their biological activities were evaluated. The binding mode of these compounds with PPARγ and α-amylase was explored using molecular docking method. The results showed that compounds 10 and 13 exhibited potent anti-diabetic and antioxidant activities.

MOLECULAR DIVERSITY (2023)

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Article Chemistry, Applied

Understanding the bioaccessibility, α-amylase and α-glucosidase enzyme inhibition kinetics of Allmania nodiflora (L.) R.Br. ex Wight polyphenols during in vitro simulated digestion

Gayathri Jagadeesan et al.

Summary: The study revealed that Allmania nodiflora leaves contain highly bioactive polyphenolics with potential medicinal value, making them suitable for the development of functional foods and nutraceutical products.

FOOD CHEMISTRY (2022)

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Article Chemistry, Applied

The inhibitory mechanism of chlorogenic acid and its acylated derivatives on α-amylase and α-glucosidase

Shan Wang et al.

Summary: This study demonstrated that the inhibitory activities of CA derivatives on enzymes were enhanced as lipophilicity increased, with C12-CA showing the strongest inhibitory effects. The effects of C12-CA on the microenvironments and secondary structures of the enzymes were greater than those of CA and other derivatives.

FOOD CHEMISTRY (2022)

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Article Chemistry, Applied

L-Arginine inhibits the activity of α-amylase: Rapid kinetics, interaction and functional implications

Yu-Tong Ye et al.

Summary: This study investigated the rapid unfolding kinetics and interaction mechanism of pancreas alpha-amylase (PPA) induced by L-arginine. The interaction between L-arginine and PPA resulted in a decrease in beta-sheet and an increase in random coil, leading to a decrease in enzyme activity. However, the unfolding of PPA could be compensated and the secondary structure change could be partially recovered by a macromolecular crowded medium. The results provide new insights into the interaction of L-arginine with digestive enzymes and the unfolding mechanism of enzymes.

FOOD CHEMISTRY (2022)

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Article Chemistry, Physical

a-Amylase inhibition, anti-glycation property and characterization of the binding interaction of citric acid with a-amylase using multiple spectroscopic, kinetics and molecular docking approaches

Oghenetega J. Avwioroko et al.

Summary: This study investigated the protective effect of citric acid as a common food preservative against the formation of advanced glycation end-products (AGEs) and its interaction with α-amylase enzyme. The results showed that citric acid inhibited α-amylase activity and suppressed AGEs formation. Citric acid interacted with α-amylase and induced conformational changes. These findings are important for the management of type 2 diabetes complications.

JOURNAL OF MOLECULAR LIQUIDS (2022)

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Article Multidisciplinary Sciences

Synthesis, α-glucosidase inhibition, α-amylase inhibition, and molecular docking studies of 3,3-di(indolyl)indolin-2-ones

Mardi Santoso et al.

Summary: The synthesized 3,3-di(indolyl)indolin-2-ones exhibited higher α-glucosidase inhibitory activities and lower α-amylase inhibitory activities. Compound 1i showed the most favorable inhibitory activity. Docking studies further supported the observed inhibitory activities. These results suggest that 3,3-di(indolyl)indolin-2-ones may serve as suitable α-glucosidase inhibitors, and the lower α-amylase activity could reduce side effects.

HELIYON (2022)

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Article Chemistry, Applied

Screening and identification of natural ?-glucosidase and ?-amylase inhibitors from partridge tea (Mallotus furetianus Muell-Arg) and in silico analysis

Songjie Li et al.

Summary: This study confirms the excellent inhibiting effects of partridge tea extracts on alpha-glucosidase and alpha-amylase, as well as its ability to promote glucose consumption in fat cells. The potential inhibitors in the tea were rapidly screened and identified using affinity ultrafiltration and mass spectrometry techniques, revealing the interactive mechanisms with the digestive enzymes.

FOOD CHEMISTRY (2022)

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Article Biochemical Research Methods

Development of a protein assay with copper chelator chromeazurol B, based on the biuret reaction

Eisaku Hokazono et al.

Summary: This study introduces a novel and highly sensitive protein assay using the biuret reaction and chromeazurol B, which shows higher molar absorption coefficient than the Lowry method. The method, named Biuret-based CAB method, is more sensitive and rapid, making it suitable for various biological samples.

ANALYTICAL BIOCHEMISTRY (2021)

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Article Biochemical Research Methods

A sensitive and high-throughput quantitative liquid chromatography high-resolution mass spectrometry method for therapeutic drug monitoring of 10 β-lactam antibiotics, linezolid and two β-lactamase inhibitors in human plasma

Sarah Van Vooren et al.

Summary: An UHPLC-HRMS method was developed for simultaneous and sensitive quantification of 10 beta-lactam antibiotics, linezolid, and beta-lactamase inhibitors in human plasma. The method showed high sensitivity and accuracy, allowing it to be suitable for routine therapeutic drug monitoring.

BIOMEDICAL CHROMATOGRAPHY (2021)

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Article Biotechnology & Applied Microbiology