期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 24, 期 22, 页码 -出版社
MDPI
DOI: 10.3390/ijms242216284
关键词
functional hyperemia; epidermal growth factor receptor (EGFR); vascular endothelial cells; phosphatidylinositol 4,5-bisphosphate (PIP2); cerebral small vessel diseases (cSVD)
Functional hyperemia, which refers to the increase in local blood perfusion in response to enhanced neuronal activity, plays a crucial role in brain health. This study found that the impairment of endothelial epidermal growth factor receptor (EGFR) signaling resulted in crippled functional hyperemia, likely due to depletion of phosphatidylinositol 4,5-bisphosphate (PIP2) and dysfunctional Kir2.1 channels in capillary endothelial cells.
Functional hyperemia-activity-dependent increases in local blood perfusion-underlies the on-demand delivery of blood to regions of enhanced neuronal activity, a process that is crucial for brain health. Importantly, functional hyperemia deficits have been linked to multiple dementia risk factors, including aging, chronic hypertension, and cerebral small vessel disease (cSVD). We previously reported crippled functional hyperemia in a mouse model of genetic cSVD that was likely caused by depletion of phosphatidylinositol 4,5-bisphosphate (PIP2) in capillary endothelial cells (EC) downstream of impaired epidermal growth factor receptor (EGFR) signaling. Here, using EC-specific EGFR-knockout (KO) mice, we directly examined the role of endothelial EGFR signaling in functional hyperemia, assessed by measuring increases in cerebral blood flow in response to contralateral whisker stimulation using laser Doppler flowmetry. Molecular characterizations showed that EGFR expression was dramatically decreased in freshly isolated capillaries from EC-EGFR-KO mice, as expected. Notably, whisker stimulation-induced functional hyperemia was significantly impaired in these mice, an effect that was rescued by administration of PIP2, but not by the EGFR ligand, HB-EGF. These data suggest that the deletion of the EGFR specifically in ECs attenuates functional hyperemia, likely via depleting PIP2 and subsequently incapacitating Kir2.1 channel functionality in capillary ECs. Thus, our study underscores the role of endothelial EGFR signaling in functional hyperemia of the brain.
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