Insights on Three Dimensional Organoid Studies for Stem Cell Therapy in Regenerative Medicine

Regenerative medicine has developed as a promising discipline that utilizes stem cells to address limitations in traditional therapies, using innovative techniques to restore and repair damaged organs and tissues. One such technique is the generation of three-dimensional (3D) organoids in stem cell therapy. Organoids are 3D constructs that resemble specific organs' structural and functional characteristics and are generated from stem cells or tissue-specific progenitor cells. The use of 3D organoids is advantageous in comparison to traditional two-dimensional (2D) cell culture by bridging the gap between in vivo and in vitro research. This review aims to provide an overview of the advancements made towards regenerative medicine using stem cells to generate organoids, explore the techniques used in generating 3D organoids and their applications and finally elucidate the challenges and future directions in regenerative medicine using 3D organoids.

Automated summary

Regenerative medicine utilizes stem cells to restore and repair damaged organs and tissues, and one important technique is the generation of three-dimensional organoids. The use of 3D organoids bridges the gap between in vivo and in vitro research, offering advantages over traditional 2D cell culture. This review provides an overview of the advancements in regenerative medicine using stem cells to generate organoids, explores the techniques and applications of 3D organoids, and discusses the challenges and future directions in this field.