期刊
NATURAL PRODUCT RESEARCH
卷 -, 期 -, 页码 -出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/14786419.2023.2289081
关键词
Edible mushroom; Auricularia auricula polysaccharide; antioxidant; structural characterisation
A new polysaccharide AAP was extracted from Auricularia auricula, showing good antioxidant activity. The purified component pAAP exhibited a unique molecular structure and special conformation.
A new polysaccharide (AAP) was extracted from Auricularia auricula by water extraction and alcohol precipitation. The antioxidant activity in vitro showed that AAP had a good scavenging effect on ABTS free radicals. Then AAP was purified by DEAE-52 ion exchange chromatography to obtain the purified component pAAP. The structure analysis showed that the molecular weight (Mw) of pAAP was 96.768 kDa, which was composed of rhamnose (Rha), arabinose (Ara), fucose (Fuc), xylose (Xyl), mannose (Man), glucose (Glu) and galactose (Gal), with the ratio of 0.1:0.157:0.33:2.797:2.881:2.988:0.587, and contained alpha-pyranose configuration and beta-pyranose configuration. Field emission scanning electron microscopy and atomic force microscopy revealed the special conformation of pAAP in the ring and chain shape. [GRAPHICS] .
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