Study on drug separation in Two-Phase aqueous systems using deep eutectic solvent consisting of choline chloride and 1,2 propanediol

Biphasic-aqueous systems have been the subject of extensive research due to their strength to simultaneously separate and purify active pharmaceutical molecules with high efficiency and purity. Aqueous Two-Phase Systems (ATPS) have been used to extract and purify cells, nucleic acids, proteins, antibodies, and tiny molecules due to their intermolecular interaction and chemical makeup. We used a deep eutectic solvent containing (choline chloride and 1,2 propanediol), K2HPO4, and water in the initial investigation of the biphasic curve of this system at the temperature of 298.15 K and atmospheric pressure. Furthermore, we used the ATPSs to study the separation of the selected pharmaceuticals. As new findings, we examined the partition coefficient and the impact of variables such as salt and the deep eutectic solvent concentration on the partitioning of the drugs. Based on the present findings, the pharmaceuticals have the propensity to be extracted in the top phase, which is rich in deep eutectic solvents. Finally, we conducted thermodynamic modeling of the present aqueous two-phase system using experimental liquid-liquid equilibrium data employing the nonrandom two-liquid nonrandom factor (NRTL-NRF) and NRTL models. The NRTL-NRF model can accurately correlate the aqueous two-phase system's thermodynamic behavior with the average relative deviation percent of 4.22.

Automated summary

Biphasic-aqueous systems have been extensively studied for their ability to simultaneously separate and purify active pharmaceutical molecules. In this study, we used a specific deep eutectic solvent to investigate the biphasic curve of the system and examined the separation of pharmaceuticals using ATPS. Our findings indicate that the pharmaceuticals preferentially partition into the top phase, which is rich in deep eutectic solvents. Additionally, we developed a thermodynamic model to describe the behavior of the aqueous two-phase system.