Quantitative assessment of phytochemicals in chickpea beverages using NIR spectroscopy

The prospects of near-infrared (NIR) spectroscopy combined with effective variable selection algorithms for quantifying phytochemical compounds in chickpea beverages were investigated in this study. As reference measurement analysis, the phytochemicals were extracted and identified via high-performance liquid chromatography. Multivariate algorithms were then applied, analyzed, and evaluated using correlation coefficients of validation set (Rp), root mean square error of prediction (RMSEP), and residual predictive deviations (RPDs). Accordingly, the competitive adaptive reweighted sampling-partial least squares (CARS-PLS) model achieved superior performance for biochanin A (Rp = 0.933, RPD = 3.63), chlorogenic acid (Rp = 0.928, RPD = 3.52), pcoumaric acid (Rp = 0.900, RPD = 2.37), and stigmasterol (Rp = 0.932, RPD = 3.15), respectively. Hence, this study demonstrated that NIR spectroscopy paired with CARS-PLS could be used for nondestructive quantitative prediction of phytochemicals in chickpea beverages during manufacture and storage.

Automated summary

This study investigated the prospects of using near-infrared (NIR) spectroscopy and effective variable selection algorithms for quantifying phytochemical compounds in chickpea beverages. The competitive adaptive reweighted sampling-partial least squares (CARS-PLS) model showed superior performance in predicting biochanin A, chlorogenic acid, pcoumaric acid, and stigmasterol.