期刊
ANALYTICA CHIMICA ACTA
卷 1285, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aca.2023.342031
关键词
Leucomalachite green; Immunochromatographic assay; Hyperbranched Au plasmonic blackbody; Fish; Shrimp
A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
A novel immunochromatographic assay (ICA) based on hyperbranched Au plasmonic blackbodies (AuPBs) with a smartphone readout was fabricated for the detection of leucomalachite green (LMG) in fish and shrimp products. The ICA was carried out in a competitive immunoassay format with AuPBs as labels. The developed AuPBs-ICA allowed for the LMG detection with a low detection limit (0.15 mu g L-1) within 5 min by the smartphone reader. With the label-AuPBs ICA, the color intensity response was linearly related to the concentrations of the LMG (0.2-1.7 mu g L-1). The test line signal could be clearly distinguished at a 1.7 mu g L-1 LMG as a cut-off level by the naked eye, which is lower than the conventional colloidal gold nanoparticle (2 mu g L-1) and star-shaped nano-particles (1.9 mu g L-1) labeling. LMG contamination in samples was determined with the proposed AuPBs-ICA and the enzyme-linked immunosorbent (ELISA). The AuPBs-ICA results showed good agreement with those from the ELISA. The proposed AuPBs-ICA has the potential to be used as a rapid, sensitive, and simple device for the analysis of LMG residues in fish and shrimp samples.
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