☆ 4.7 Review

Has the time finally come for green oleochemicals and biodiesel production using large-scale enzyme technologies? Current status and new developments

BIOTECHNOLOGY ADVANCES (2023)

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Article Biotechnology & Applied Microbiology

Synthesis of organic-inorganic hybrid nanoflowers of lipases from Candida antarctica type B (CALB) and Thermomyces lanuginosus (TLL): Improvement of thermal stability and reusability

Isabela Oliveira Costa et al.

Summary: Enzyme immobilization is a method to enhance enzyme application by allowing their reuse and improving stability. The immobilization of enzymes through nanoflowers structures is an innovative and cost-effective technique. This study aims to synthesize hybrid biocatalytic nanostructures similar to flowers using lipases from Candida antarctica type B (CALB) and Thermomyces lanuginosus (TLL). The production of nanoflowers was achieved through precipitation of lipases with CuCl2 or CuSO4 salts for 72 hours. However, challenges were faced in terms of low thermal stability and reusability. Glutaraldehyde cross-linking was tested to overcome these challenges, resulting in improved thermal stability and efficiency.

ENZYME AND MICROBIAL TECHNOLOGY (2023)

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Article Chemistry, Applied

Biomass-derived hydrophobic metal-organic frameworks solid acid for green efficient catalytic esterification of oleic acid at low temperatures

Yuncong Li et al.

Summary: In this study, a renewable solid acid catalyst FDCA/SA-Hf with a high degree of hydrophobicity was prepared using biomass-based materials. FDCA/SA-Hf showed efficient performance in producing biodiesel from free fatty acids and nonedible raw oils. It exhibited stable structural properties and excellent hydrophobic network, enabling high biodiesel yield and good reusability. Therefore, FDCA/SA-Hf has great potential in the field of heterogeneous acid-mediated catalysis, especially for biodiesel production.

FUEL PROCESSING TECHNOLOGY (2023)

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Review Biochemistry & Molecular Biology

Structural features, temperature adaptation and industrial applications of microbial lipases from psychrophilic, mesophilic and thermophilic origins

Gulam Rabbani et al.

Summary: Microbial lipases are excellent biocatalysts that can catalyze various reactions in both aqueous and non-aqueous media. This review focuses on microbial lipases from different sources (psychrophiles, mesophiles, and thermophiles), highlighting the structural diversity in temperature adaptation and industrial applications. Understanding the molecular mechanisms of temperature adaptation in lipases is of great interest, as extremophilic lipases are widely used in biotransformation reactions with higher yields and unique products.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2023)

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Article Chemistry, Physical

Recent advances in enzyme immobilization based on nanoflowers

Kang Xu et al.

Summary: Enzyme immobilization technologies aim to create reusable enzymes with improved properties for industrial applications. However, traditional immobilization methods often result in reduced enzyme activity compared to free enzymes. The recently developed hierarchical Nanoflowers (NFs) technology offers a promising alternative with enhanced loading capacity and high specific surface area. Recent research has demonstrated its cost-effectiveness and environmental friendliness in enzyme immobilization. This review focuses on the recent advances and applications of environmentally benign hierarchical NFs in enzyme immobilization.

JOURNAL OF CATALYSIS (2023)

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Article Chemistry, Physical

Technical-Economic Assessment-The Missing Piece for Increasing the Attractiveness of Applied Biocatalysis in Ester Syntheses?

Ronaldo Rodrigues de Sousa et al.

Summary: Although significant advances have been made in the biocatalytic synthesis of esters, few industrial plants worldwide currently produce esters using biocatalysts. Biocatalytic routes can provide green and sustainable esters with operational advantages over conventional syntheses. However, most literature neglects or generically describes the economic issues, and scaling-up studies are scarce. The cost of immobilized lipases, the main disadvantage of biocatalysis, has not been studied as extensively as other technical aspects. Preliminary techno-economic assessments are crucial to overcome the economic drawbacks and promote wider application of biocatalytic ester syntheses in large-scale contexts.

CATALYSTS (2023)

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Article Biotechnology & Applied Microbiology

Optimization of low-cost solid-state fermentation media for the production of thermostable lipases using agro-industrial residues as substrate in culture of Bacillus amyloliquefaciens

Haniya Mazhar et al.

Summary: In this study, a low-cost solid-state fermentation media was optimized for the production of thermostable lipases in Bacillus amyloliquefaciens culture. Cheap agro-industrial wastes were used to enhance lipase production. The obtained results showed that agro-industrial wastes are the cheapest sources for low-cost lipase production.

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY (2023)

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Article Biotechnology & Applied Microbiology

Evaluation of olive mill wastewater as culture medium to produce lipolytic enzymes by Bacillus aryabhattai BA03

Alicia Paz et al.

Summary: In this study, olive mill wastewater (OMWW) was used as a low-cost substrate to produce lipases by Bacillus aryabhattai BA03, reducing the production costs. Non-sterilized or sterilized media formulated with 25% OMWW showed double or triple the enzyme activity compared to synthetic medium, while the highest activity was observed at pH 8, 27 degrees C, and 50% OMWW under non-sterile conditions.

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY (2023)

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Article Biophysics

Nanozymes and nanoflower: Physiochemical properties, mechanism and biomedical applications

Mohammad Perwez et al.

Summary: In recent years, researchers have developed enzyme mimicking nanomaterials and enzymatic hybrid nanoflower as alternatives to natural enzymes. These nanozymes and organic inorganic hybrid nanoflower possess various enzyme mimicking activities, enhanced catalytic activities, low cost, easy preparation, stability and biocompatibility. This review compares nanozymes and hybrid nanoflower in terms of physiochemical properties, common synthetic routes, mechanism of action, modification, green synthesis and application in disease diagnosis, imaging, environmental remediation and disease treatment. It also discusses the current challenges and potential future developments in nanozyme and hybrid nanoflower research.

COLLOIDS AND SURFACES B-BIOINTERFACES (2023)

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Article Biotechnology & Applied Microbiology

Simultaneous lipase production and immobilization: morphology and physiology study of Penicillium simplicissimum in submerged and solid-state fermentation with polypropylene as an inert support.

Jaqueline Greco-Duarte et al.

Summary: The study investigated the influence of different carbon sources (glucose, olive oil, and a combination of both) on the physiology and morphology of Penicillium simplicissimum through submerged and solid-state fermentations. Results showed that the fungus exhibited different morphologies in different cultivation conditions and media. Furthermore, it was found that the stress conditions in solid-state fermentation may be the key to achieving higher biomass and lipase productivity. The developed process of simultaneous production and immobilization of lipase shows potential as a biocatalyst without the need for downstream processes.

ENZYME AND MICROBIAL TECHNOLOGY (2023)

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Review Environmental Sciences

Sustainable biofuel economy: A mapping through bibliometric research

Morshadul Hasan et al.

Summary: This study conducts a bibliometric analysis of biofuel economic research and finds that biofuels are linked to a sustainable economy with impacts on poverty reduction, agriculture development, renewable energy production, economic growth, climate change policy, and environmental protection.

JOURNAL OF ENVIRONMENTAL MANAGEMENT (2023)

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Article Energy & Fuels

Efficient delignification of wheat straw for microbial lipid production enabled by a novel ternary deep eutectic solvent containing ethylene glycol

Jinyu Tan et al.

Summary: A novel ternary deep eutectic solvent (DES) was designed for wheat straw pretreatment, which achieved non-condensed lignin and high-yield fermentable sugars. The introduction of ethylene glycol into the DES protected lignin structure and improved enzymatic digestibility of cellulose. Trichosporon cutaneum cultivated in the waste liquid and residue hydrolysate produced 8.7 g microbial lipid per 100 g wheat straw, surpassing previous reports.

FUEL (2023)

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Article Chemistry, Applied

Bifunctional acid-activated montmorillonite catalyzed biodiesel production from non-food oil: Characterization, optimization, kinetic and thermodynamic studies

Long Chen et al.

Summary: In this study, a novel bifunctional catalytic material 30%Sn-MMT-SO3H was fabricated and successfully applied in converting Jatropha curcas oil into biodiesel. The material showed high activity, reusability, and promising prospects for industrialization of biodiesel production.

FUEL PROCESSING TECHNOLOGY (2023)

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Article Green & Sustainable Science & Technology

Applications of nanotechnology in biodiesel combustion and post-combustion stages

Homa Hosseinzadeh-Bandbafha et al.

Summary: Diesel fuel is efficient, durable, and profitable, but it is a major source of airborne pollutants. Biodiesel has been developed as a potential replacement, but its combustion has drawbacks, especially in nitrogen oxide emissions. Nanotechnology has shown promise in improving biodiesel combustion and reducing emissions, but there are challenges to overcome.

RENEWABLE & SUSTAINABLE ENERGY REVIEWS (2023)

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Review Chemistry, Multidisciplinary

Nanoflowers: A New Approach of Enzyme Immobilization

Felipe Pereira da Costa et al.

Summary: Enzymes are biocatalysts known for their versatility, selectivity, and favorable operating conditions compared to chemical catalysts. However, their large-scale application is limited by high costs and low stability under extreme reaction conditions. Immobilization techniques can solve these problems, and organic-inorganic hybrid nanoflowers have shown advantages such as high surface area, simplicity of synthesis, high stability, and catalytic activity in enzymatic catalysis.

CHEMICAL RECORD (2022)

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Article Green & Sustainable Science & Technology

How the biodiesel from immobilized enzymes production is going on: An advanced bibliometric evaluation of global research

Luciana da Silva Dutra et al.

Summary: The study conducted an in-depth analysis of global scientific production on biodiesel production through immobilized enzymes, revealing widespread international collaboration and interest in this field, with China, Brazil, and India emerging as key countries in research and development.

RENEWABLE & SUSTAINABLE ENERGY REVIEWS (2022)

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Article Food Science & Technology

Microbial lipases: Propitious biocatalysts for the food industry

Cleonice Aparecida Salgado et al.

Summary: Microbial lipases are important enzymes with various advantages and wide applications mainly in the food industry. Researchers are exploring the use of low-cost agro-industrial residues for lipase production to improve yield and characteristics, as well as applying protein engineering techniques and immobilization methods.

FOOD BIOSCIENCE (2022)

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Article Energy & Fuels

A two-phase model for simulation of water transfer during lipase production by solid-state cultivation in a tray bioreactor using babassu residues as substrate

Mirella P. Diniz da Silva et al.

Summary: This research modeled the water transfer during lipases production by solid-state cultivation using babassu coconut bagasse as substrate. The results showed that the moisture content of the solid matrix is greatly influenced by the relative humidity of the inlet air, and the fungal growth is limited by its water activity. The proposed model can guide the scale-up of bioreactors.

CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION (2022)

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Review Chemistry, Applied

Recent development of advanced processing technologies for biodiesel production: A critical review

Muhammad Aamir Bashir et al.

Summary: Research in biodiesel production technologies is focused on improving efficiency through the use of various catalysts and feedstocks. Emerging technologies like enzyme catalysis, batch reactors, and continuous flow reactors show promise in achieving high conversion yields.

FUEL PROCESSING TECHNOLOGY (2022)

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Article Chemistry, Physical

Synthesis and characterization of new bifunctional SnZrSi oxide catalysts for biodiesel production

Shaimaa M. Ibrahim et al.

Summary: A set of unprecedented mesoporous SnO2/ZrSiO4 solids were synthesized through ultrasound-assisted co-precipitation. The impact of silica dopant content on the characterization evolution of the sonicated ZrSnO4 interface and biodiesel production efficiency was examined. The results showed the formation of reconstructed rutile-SnO4 and zircon as new active phases, improved morphology and interesting optical behavior, and different acidic properties. The optimal catalyst exhibited the highest yield for esterification of palmitic acid, while a different catalyst showed the highest yield for transesterification of soybean oil.

JOURNAL OF MOLECULAR LIQUIDS (2022)

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Review Biochemistry & Molecular Biology

Applications of immobilized lipases in enzymatic reactors: A review

Daniela Remonatto et al.

Summary: Lipases are efficient biocatalysts with wide applications in various industries. Immobilization of lipases enhances their stability, selectivity, and half-life. However, achieving high catalytic efficiency in immobilized lipase reactions requires optimization of reaction conditions and operational characteristics.

PROCESS BIOCHEMISTRY (2022)

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Article Environmental Sciences

Circular Economy Applied to Organic Residues and Wastewater: Research Challenges

Benedicte Bakan et al.

Summary: The article discusses the importance of turning organic residues and wastewater into valuable assets and presents key directions and challenges for driving the development of circular bioeconomy solutions. By proposing innovative processes, establishing multi-scale cross-sectoral organizations, and enhancing research-legislation interactions, the resourceful utilization of organic residues and wastewater can be achieved.

WASTE AND BIOMASS VALORIZATION (2022)

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Review Engineering, Environmental

A review on the utility of microbial lipases in wastewater treatment

Vijay D. Nimkande et al.

Summary: Microbial lipases have immense potential in wastewater treatment as an attractive alternative to traditional physico-chemical methods. This article discusses the limited studies on the use of lipases in wastewater treatment and highlights the need for further research in this area.

JOURNAL OF WATER PROCESS ENGINEERING (2022)

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Article Chemistry, Multidisciplinary

Selective synthesis of alpha monoglycerides by a clean method: Techno-economic and environmental assessment

Ahmad Mustafa et al.

Summary: This study proposes an alternative green and selective biocatalytic route for the production of Glycerin Monostearate. The proposed enzymatic method achieves high yield of alpha-monostearin at a lower temperature compared to the conventional method, avoiding energy consumption and color darkening issues. Economic and environmental analyses show that the proposed ENZ process has lower capital investment and carbon footprint.

SUSTAINABLE CHEMISTRY AND PHARMACY (2022)

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Article Biochemistry & Molecular Biology

Stabilization of immobilized lipases by treatment with metallic phosphate salts

Jose R. Guimaraes et al.

Summary: In this study, lipases from different sources were immobilized on octylagarose beads and treated with phosphate and/or metallic salts. The effects of phosphate/metal modification on enzyme features varied depending on the loading of the support. Some enzymes showed improved stability, while others showed increased activity.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022)

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Review Engineering, Chemical

Antimicrobial Properties of Lauric Acid and Monolaurin in Virgin Coconut Oil: A Review

Febri Odel Nitbani et al.

Summary: Virgin coconut oil, obtained through wet processing, contains high levels of lauric acid, which has antimicrobial properties. It is widely recognized as a healthy food due to its antibacterial, antifungal, and antiviral effects.

CHEMBIOENG REVIEWS (2022)

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Review Energy & Fuels

Challenges and perspectives on innovative technologies for biofuel production and sustainable environmental management

Shamshad Khan et al.

Summary: This article discusses the increase in demand for fossil fuels caused by human activities and the severe environmental problems that result from it. The study concludes that bioenergy is a green alternative that can be used for diverse energy needs, reducing the use of fossil fuels. Producing biofuels from energy crops and microalgae is identified as the most efficient and attractive method. The article also highlights the role of nanotechnology in biofuel production.

FUEL (2022)

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Article Chemistry, Applied

Improved Synthesis of α-Glycerol Monolaurate Using Lipozyme TL IM

Febri Odel Nitbani et al.

Summary: This study successfully synthesized ??-Glycerol Monolaurate through a two-step reaction. The reaction showed high yield and purity, making it suitable for industrial production.

JOURNAL OF OLEO SCIENCE (2022)

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Article Green & Sustainable Science & Technology

Green synthesis of isopropyl palmitate using immobilized Candida antarctica lipase: Process optimization using response surface methodology

Ahmad Mustafa et al.

Summary: This study aims to produce isopropyl palmitate (IPP) in a solvent-free system. The esterification reaction between isopropyl alcohol and palmitic acid was performed using immobilized Candida antarctica lipase as a biocatalyst. The optimal reaction conditions were determined using response surface methodology, and a process flow diagram for semicontinuous production of IPP was suggested.

CLEANER ENGINEERING AND TECHNOLOGY (2022)

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Article Chemistry, Applied

Immobilization of lipases on lignocellulosic bamboo powder for biocatalytic transformations in batch and continuous flow

Bruna G. Palma et al.

Summary: The study using bamboo powder as a support for immobilizing two different biocatalysts in ethyl palmitate synthesis showed that both IB-CalB and IB-RM exhibited good recycling efficiency. IB-CalB demonstrated higher enantioselectivity conversion. The high conversion rate of IB-CalB in enantioselective catalysis under flow mode regime indicates the potential of a simple and cost-effective enzyme adsorption method for industrial biocatalytic transformation.

CATALYSIS TODAY (2021)

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Review Biotechnology & Applied Microbiology

Enzyme co-immobilization: Always the biocatalyst designers' choice ... or not? ...

Sara Arana-Pena et al.

Summary: Enzyme co-immobilization offers kinetic advantages but also drawbacks, such as the need to discard the entire biocatalyst if one enzyme becomes inactivated. The co-immobilization of cofactors and enzymes is of particular interest for the construction of artificial cells and complex one-pot transformations.

BIOTECHNOLOGY ADVANCES (2021)

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Article Energy & Fuels

Glycerolysis treatment to enhance biodiesel production from low-quality feedstocks

Abdallah S. Elgharbawy et al.

Summary: The depletion of diesel fuel and pollution problems lead to the search for alternative energy sources, with biodiesel being the most important one. The high cost of feedstock is the main obstacle to the commercialization of biodiesel. Glycerolysis treatment can effectively reduce FFAs content in low-quality feedstock, making it suitable for transesterification.

FUEL (2021)

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Article Biochemistry & Molecular Biology

Application of Rhizomucor miehei lipase-displaying Pichia pastoris whole cell for biodiesel production using agro-industrial residuals as substrate

Raphael Oliveira Sena et al.

Summary: This study successfully utilized a novel biocatalyst RML-PIR1 for producing biodiesel from residual agro-industrial fatty acids. The biocatalyst demonstrated optimal activity at 45 degrees C, with high stability and enhanced reuse potential through treatment with GA and PEG. This new biocatalyst shows promising potential for biotechnological applications, particularly in large-scale biofuel production.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)

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Article Chemistry, Physical

Novel SnZr oxides nanomaterials synthesized by ultrasonic-assisted co-precipitation method: Application in biodiesel production and DFT study

Shaimaa M. Ibrahim et al.

Summary: Novel Sn2O3-ZrSnO4 nanoparticles were prepared by ultrasonic-assisted co-precipitation method for biodiesel production through esterification reaction. The optimized catalyst, SnZr15m, showed 88.5% stearic acid conversion under optimum conditions, with theoretical and practical analysis of the kinetic and thermodynamic parameters of the reaction carried out.

JOURNAL OF MOLECULAR LIQUIDS (2021)

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Article Biotechnology & Applied Microbiology

Insights into media supplementation in solid-state fermentation of soybean hulls by Yarrowia lipolytica: Impact on lipase production in tray and insulated packed-bed bioreactors

Felipe do Nascimento et al.

Summary: Solid-state fermentation with Yarrowia lipolytica requires appropriate medium supplementation and bioreactor configuration for efficient lipase production. Supplementation with complex medium can enhance lipase activity, while the carbon source and temperature increase have impacts on lipase production during scaling-up of SSF.

BIOCHEMICAL ENGINEERING JOURNAL (2021)

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Article Biochemistry & Molecular Biology

Current perspectives for microbial lipases from extremophiles and metagenomics

Swati Verma et al.

Summary: Microbial lipases are widely used biocatalysts for environmental and industrial applications. Extremophile lipases exhibit higher activity and diversity, allowing for the design of biotransformation reactions with higher yields and less byproducts. Additionally, novel lipases can be discovered through analysis of unexplored nucleotide/metagenomic databases.

BIOCHIMIE (2021)

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Review Food Science & Technology

Biopolymers and nanostructured materials to develop pectinases-based immobilized nano-biocatalytic systems for biotechnological applications

Shuangshuang Zhang et al.

Summary: Pectinases are emerging enzymes in the biotechnology industry, with diverse industrial applications. Enzyme immobilization has become a promising approach to address challenges such as low operational stability and recyclability, leading to improved enzyme performance and commercial feasibility. Immobilization of pectinases using polymers and nanostructured materials has shown to enhance their thermal stability and catalytic characteristics for industrial exploitability.

FOOD RESEARCH INTERNATIONAL (2021)

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Review Energy & Fuels

Biomass-based biorefineries: An important architype towards a circular economy

Bikash Kumar et al.

Summary: Bio-based biorefineries provide a potential alternative to fossil-based resources, utilizing natural organic biomass for biofuel and biochemical production at a large scale. The integration of existing technologies can facilitate sustainable development and economic growth, with biomass as a abundant and technologically advanced resource for the future.

FUEL (2021)

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Article Chemistry, Multidisciplinary

Enzymatic esterification of lauric acid to give monolaurin in a microreactor

Shanshan Miao et al.

Summary: This paper presents a new method for synthesizing monolaurin using a microreactor to catalyze the esterification between lauric acid and glycerol. The method shows higher conversion and selectivity compared to traditional batch methods, with potential applications to other multiphase and enzymatic reactions.

JOURNAL OF CHEMICAL RESEARCH (2021)

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Article Engineering, Chemical

Recent Advances in Feedstock and Lipase Research and Development towards Commercialization of Enzymatic Biodiesel

Vasudeo Zambare et al.

Summary: This work reviews the recent progress in feedstock development for biodiesel production, lipase research, and development, as well as current challenges and opportunities for biodiesel commercialization from technological, economic, and social perspectives. It critically discusses biodiesel producers, markets, challenges, and opportunities, including environmental considerations.

PROCESSES (2021)

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Article Chemistry, Physical

Synthesis of lipase/silica biocatalysts through the immobilization of CALB on porous SBA-15 and their application on the resolution of pharmaceutical derivatives and on nutraceutical enrichment of natural oil

Eliane Pereira Cipolatti et al.

Summary: A recombinant lipase from Candida antarctica was immobilized on mesoporous silica particles and used for producing food oils enriched with PUFA and resolving chiral myo-inositol derivatives. Economic analysis showed the feasibility of enzymatic production of such compounds.

MOLECULAR CATALYSIS (2021)

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Article Energy & Fuels

Lipase catalyzed solvent free synthesis of monoacylglycerols in various reaction systems and coupling reaction with pervaporation for in situ water removal

Yamini Satyawali et al.

Summary: This work presents a practical comparison of simple reaction systems for enzymatic production of MAG, focusing on water removal strategies and product selectivity. High fatty acid conversion was achieved in all reactor systems, with product composition and selectivity influenced by reaction conditions and setups. The study demonstrated the practical applicability of packed bed reactor coupled with pervaporation for enzyme reuse and in situ water removal using zeolite based membranes.

CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION (2021)

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Article Energy & Fuels

Acai seed ash as a novel basic heterogeneous catalyst for biodiesel synthesis: Optimization of the biodiesel production process

Erica Karine Lourenco Mares et al.

Summary: The study explored the catalytic potential of acai seed ash for biodiesel production through methyl transesterification of soybean oil. The optimal conditions produced biodiesel with an ester content of 98.5%. The catalyst exhibited high metal oxide content and basic surface sites, leading to efficient biodiesel production. Reusing the catalyst showed stable performance with ester content above 80.0% after regeneration.

FUEL (2021)

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Article Biotechnology & Applied Microbiology

Use of different kinds of wastes for lipase production: Inductive effect of waste cooking oil on activity

Seyda Cagatay et al.

Summary: This study evaluated the effects of by-product molasses and waste orange peels as nutrient medium, and the inductive effect of waste cooking oil on lipase production with Rhizopus arrhizus. The results showed that the highest specific growth rate and maximum biomass concentration were achieved using WOP, while WCO enhanced intracellular lipase activity but diminished extracellular activity. Overall, the study concludes that lipase production can be optimized using WOP and WCO.

JOURNAL OF BIOSCIENCE AND BIOENGINEERING (2021)

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Review Chemistry, Physical

Trends in Widely Used Catalysts for Fatty Acid Methyl Esters (FAME) Production: A Review

Shafaq Nisar et al.

Summary: The production of fatty acid methyl esters requires the use of cost-effective, environmentally friendly, and effective catalysts. Currently, alkaline and acidic catalysts in both homogeneous and heterogeneous phases are widely used. Promising catalysts include heteropoly acids, polyoxometalate compounds, carbonaceous materials, and vanadium phosphate. Biocatalysts, especially lipase, are also gaining attention for large-scale FAME production. Future directions include exploring the possibilities of these catalysts in biodiesel production.

CATALYSTS (2021)

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Article Biochemistry & Molecular Biology

Lipase-Catalyzed Esterification of Geraniol and Citronellol for the Synthesis of Terpenic Esters

Layane da Silva Correa et al.

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2020)

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Article Green & Sustainable Science & Technology

A cleaner enzymatic approach for producing non-phthalate plasticiser to replace toxic-based phthalates

Hadeel Hosney et al.

CLEAN TECHNOLOGIES AND ENVIRONMENTAL POLICY (2020)

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Article Biochemistry & Molecular Biology

Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering

Sara Arana-Pena et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)

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Article Biochemistry & Molecular Biology

Sequential optimization and large scale production of lipase using tri-substrate mixture from Aspergillus niger MTCC 872 by solid state fermentation

Venkatesh Mandari et al.

PROCESS BIOCHEMISTRY (2020)

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Article Biochemistry & Molecular Biology

Interesterification of Soybean Oil with Propylene Glycol in Supercritical Carbon Dioxide and Analysis by NMR Spectroscopy

Nazanin Vafaei et al.

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2020)

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Article Energy & Fuels

Optimization of Aspergillus niger lipase production by solid state fermentation of agro-industrial waste

Dwini Normayulisa Putri et al.

ENERGY REPORTS (2020)

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Article Chemistry, Physical

G_Fe3O4/Ag supporting Candida rugosa lipase for the green synthesis of pomegranate seed oil derived liquid wax esters

Maria Sarno et al.

APPLIED SURFACE SCIENCE (2020)

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Article Agricultural Engineering

One-step hydrothermal synthesis of magnetic rice straw for effective lipase immobilization and its application in esterification reaction

Sachin V. Otari et al.

BIORESOURCE TECHNOLOGY (2020)

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Article Engineering, Environmental

Solving the H2O2 by-product problem using a catalase-mimicking nanozyme cascade to enhance glycolic acid oxidase

Mengzhao Jiao et al.

CHEMICAL ENGINEERING JOURNAL (2020)

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Article Chemistry, Applied

Trace water activity could improve the formation of 1,3-oleic-2-medium chain-rich triacylglycerols by promoting acyl migration in the lipase RM IM catalyzed interesterification

Bin Peng et al.

FOOD CHEMISTRY (2020)

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Review Chemistry, Physical

Recent Advances in Enzyme-Nanostructure Biocatalysts with Enhanced Activity

Jing An et al.

CATALYSTS (2020)

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Review Biochemistry & Molecular Biology

Production and use of immobilized lipases in/on nanomaterials: A review from the waste to biodiesel production

Le Zhong et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)

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Review Chemistry, Multidisciplinary

Developments in the Use of Lipase Transesterification for Biodiesel Production from Animal Fat Waste

Fidel Toldra-Reig et al.

APPLIED SCIENCES-BASEL (2020)

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Review Biotechnology & Applied Microbiology

Lipase immobilization on ceramic supports: An overview on techniques and materials

Jessica Mulinari et al.

BIOTECHNOLOGY ADVANCES (2020)

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Review Biotechnology & Applied Microbiology

Microbial lipases and their industrial applications: a comprehensive review

Prem Chandra et al.

MICROBIAL CELL FACTORIES (2020)

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Review Chemistry, Physical

Advances in Recombinant Lipases: Production, Engineering, Immobilization and Application in the Pharmaceutical Industry

Fabiano Jares Contesini et al.

CATALYSTS (2020)

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Article Engineering, Chemical

A Multi-Scale Approach to Modeling the Interfacial Reaction Kinetics of Lipases with Emphasis on Enzyme Adsorption at Water-Oil Interfaces

Sherly Rusli et al.

PROCESSES (2020)

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Article Agricultural Engineering

Experimental study and preliminary economic evaluation of enzymatic biodiesel production by an integrated process using co-products from palm (Elaeais guineensis Jaquim) industry

Ana Cristina A. Collaco et al.

INDUSTRIAL CROPS AND PRODUCTS (2020)

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Kinetics and Gibbs Function Studies on Lipase-Catalyzed Production of Non-Phthalate Plasticizer

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Semi-continuous Production of 2-Ethyl Hexyl Ester in a Packed Bed Reactor: Optimization and Economic Evaluation

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Mango agro-industrial wastes for lipase production from Yarrowia lipolytica and the potential of the fermented solid as a biocatalyst

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ACS CATALYSIS (2019)

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Increasing the Enzyme Loading Capacity of Porous Supports by a Layer-by-Layer Immobilization Strategy Using PEI as Glue

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Synthesis of catalase-inorganic hybrid nanoflowers via sonication for colorimetric detection of hydrogen peroxide

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Biodiesel production catalyzed by liquid and immobilized enzymes: Optimization and economic analysis

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Immobilization of Rhizomucor miehei lipase onto the organic functionalized SBA-15: Their enzymatic properties and glycerolysis efficiencies for diacylglycerols production

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Production of lipases in cottonseed meal and application of the fermented solid as biocatalyst in esterification and transesterification reactions

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Highly Selective Synthesis of Monolaurin via Enzymatic Transesterification under Batch and Continuous Flow Conditions

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Solid-state fermentation of co-products from palm oil processing: Production of lipase and xylanase and effects on chemical composition

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Bacterial lipases: A review on purification and characterization

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Extraction of fresh banana waste juice as non-cellulosic and non-food renewable feedstock for direct lipase production

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Pilot-scale development of core-shell polymer supports for the immobilization of recombinant lipase B from Candida antarctica and their application in the production of ethyl esters from residual fatty acids

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Integrated Process for the Enzymatic Production of Fatty Acid Sugar Esters Completely Based on Lignocellulosic Substrates

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Adding value to the oil cake as a waste from oil processing industry: Production of lipase in solid state fermentation

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Optimization of lipase production from organic solid waste by anaerobic digestion and its application in biodiesel production

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Highly selective biocatalytic synthesis of monoacylglycerides in sponge-like ionic liquids

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Converting oils high in phospholipids to biodiesel using immobilized Aspergillus oryzae whole-cell biocatalysts expressing Fusarium heterosporum lipase

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Operational stabilities of different chemical derivatives of Novozym 435 in an alcoholysis reaction

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Optimisation and economic assessment of lipase-catalysed production of monoesters using Rhizomucor miehei lipase in a solvent-free system

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Engineering and application of enzymes for lipid modification, an update

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Efficient solvent-less separation of lipids from municipal wet sewage scum and their sustainable conversion into biodiesel

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Synthesis of ethyl caprylate in organic media using Candida rugosa lipase immobilized on exfoliated graphene oxide: Process parameters and reusability studies

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Evaluation of different methods for immobilization of Candida antarctica lipase B (CalB lipase) in polyurethane foam and its application in the production of geranyl propionate

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Significant enhancement of Pseudomonas aeruginosa FW_SH-1 lipase production using response surface methodology and analysis of its hydrolysis capability

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Integrated Biocatalysis in Multistep Drug Synthesis without Intermediate Isolation: A de Novo Approach toward a Rosuvastatin Key Building Block

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Accurel MP 1000 as a support for the immobilization of lipase from Burkholderia cepacia: Application to the kinetic resolution of myo-inositol derivatives

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PROCESS BIOCHEMISTRY (2015)

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Article Biotechnology & Applied Microbiology

Utilization of agro-industrial wastes for the production of lipase from Stenotrophomonas maltophilia isolated from Arctic and optimization of physical parameters

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Efficient conversion of brown grease produced by municipal wastewater treatment plant into biofuel using aluminium chloride hexahydrate under very mild conditions

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Article Biochemistry & Molecular Biology

Preparation of core-shell polymer supports to immobilize lipase B from Candida antarctica Effect of the support nature on catalytic properties

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Oleochemical Industry Future through Biotechnology

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Heterofunctional Hydrophilic Hydrophobic Porous Silica as Support for Multipoint Covalent Immobilization of Lipases: Application to Lactulose Palmitate Synthesis

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Microbial Enzymes: Tools for Biotechnological Processes

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Enzymatic biodiesel production: An overview of potential feedstocks and process development

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BIORESOURCE TECHNOLOGY (2013)

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Utilization of oleochemical industry residues as substrates for lipase production for enzymatic sunflower oil hydrolysis

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JOURNAL OF CLEANER PRODUCTION (2013)

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An improved method for the synthesis of 1-monoolein

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Kinetic resolution of a precursor for myo-inositol phosphates under continuous flow conditions

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Geranyl acetate synthesis catalyzed by Thermomyces lanuginosus lipase immobilized on electrospun polyacrylonitrile nanofiber membrane

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Review Biochemistry & Molecular Biology

Overview of Fungal Lipase: A Review

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Protein-inorganic hybrid nanoflowers

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Lipase production: An insight in the utilization of renewable agricultural residues

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Hydrolysis of vegetable oils and fats to fatty acids over solid acid catalysts

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APPLIED CATALYSIS A-GENERAL (2011)

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Study of biodiesel production from animal fats with high free fatty acid content

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BIORESOURCE TECHNOLOGY (2011)

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Simple and efficient immobilization of lipase B from Candida antarctica on porous styrene-divinylbenzene beads

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Article Biochemistry & Molecular Biology

Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium

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Lipase B from Candida antarctica immobilized on octadecyl Sepabeads: A very stable biocatalyst in the presence of hydrogen peroxide

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PROCESS BIOCHEMISTRY (2011)

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Article Biochemistry & Molecular Biology

Effects of the combined use of Thermomyces lanuginosus and Rhizomucor miehei lipases for the transesterification and hydrolysis of soybean oil

Rafael C. Rodrigues et al.

PROCESS BIOCHEMISTRY (2011)

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Biodiesel production with immobilized lipase: A review

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Enzymatic synthesis of monoglycerides by esterification reaction using Penicillium camembertii lipase immobilized on epoxy SiO2-PVA composite

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Perspectives on biodiesel as a sustainable fuel

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Political, economic and environmental impacts of biofuels: A review

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Mesoporous materials for encapsulating enzymes

Chia-Hung Lee et al.

NANO TODAY (2009)

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Meeting Abstract Biochemical Research Methods

Development and operation of a multi-proposal solid-state fermentation bioreactor for lipase production using an agro-industrial residue as culture medium

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NEW BIOTECHNOLOGY (2009)

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Efficient production of biodiesel from high free fatty acid-containing waste oils using various carbohydrate-derived solid acid catalysts

Wen-Yong Lou et al.

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Alteration of lipase properties by protein engineering methods

Uwe T. Bornscheuer

OCL-OILSEEDS AND FATS CROPS AND LIPIDS (2008)

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Studies on protein adsorption in a vortex flow reactor

Maria J. Ibanez-Gonzalez et al.

PROCESS BIOCHEMISTRY (2007)

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Effect of the immobilization protocol in the activity, stability, and enantioslectivity of Lecitase® Ultra

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Review Chemistry, Applied

Enzyme immobilization: The quest for optimum performance

Roger A. Sheldon

ADVANCED SYNTHESIS & CATALYSIS (2007)

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Enzymatic synthesis of glyceride esters in solvent-free system: Influence of the molar ratio, lipase source and functional activating agent of the support

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Enzymatic hydrolysis of castor oil: Process intensification studies

Meenal S. Puthli et al.

BIOCHEMICAL ENGINEERING JOURNAL (2006)

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Article Biochemical Research Methods

Preparation of cross-linked enzyme aggregates by using bovine serum albumin as a proteic feeder

S Shah et al.

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Enzymatic synthesis of partial glycerol caprate in solvent-free media

YM Xia et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2003)

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Article Biochemistry & Molecular Biology

Interfacial adsorption of lipases on very hydrophobic support (octadecyl-Sepabeads):: immobilization, hyperactivation and stabilization of the open form of lipases

JM Palomo et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2002)

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Biodiesel production from crude palm oil and evaluation of butanol extraction and fuel properties

E Crabbe et al.

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Review Biochemistry & Molecular Biology

Improved biocatalysts by directed evolution and rational protein design

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CURRENT OPINION IN CHEMICAL BIOLOGY (2001)

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