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Has the time finally come for green oleochemicals and biodiesel production using large-scale enzyme technologies? Current status and new developments

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BIOTECHNOLOGY ADVANCES
卷 69, 期 -, 页码 -

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PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2023.108275

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Biodiesel; Oleochemicals; Lipases; Fatty acids; Fatty esters; Monoglycerides; Immobilization

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With the growth of the chemical industry, the demand for cheaper and more environmentally friendly alternatives to petrochemicals has increased. Oleochemicals and biodiesel are considered as green alternatives to petroleum derivatives. The enzymatic production of these compounds is more environmentally friendly and energy efficient compared to traditional chemical processes. However, the high cost of lipases has limited their large-scale utilization. This review discusses the factors affecting the economic feasibility of enzymatic processes and reports the recent advances in lipase-catalyzed production of these compounds in both lab and large-scale settings.
With the growth of the chemical industry over the last decade, the need for cheaper (and more environmentally friendly) alternatives to petrochemicals of ever-increasing cost has grown steadily. Oleochemicals and biodiesel (OC/BD) are considered as green alternatives to petroleum derivatives, because they come from renewable oils and fats. OC/BD are currently produced by the traditional energy intensive chemical catalyzed methods, which have several economic and environmental drawbacks. For these reasons, the enzymatic production of OC/BD has attracted a growing attention for their greener pathway with respect to the chemically catalyzed processes. Lipase-catalyzed processes have a low energy requirement, since reactions are performed under atmospheric pressure and mild temperature and without the creation of side reactions. Furthermore, utilization of enzyme catalysts offers many advantages such as reducing the initial capital investment due to simplified downstream processing steps. Despite all the previous advantages, however, the high cost of lipases restricted their large-scale utilization. In the past decade, efforts have been made to reduce the cost of the enzymatic-catalyzed synthesis of OC/BD. However, most previous studies have studied only the technical feasibility of the lipase-catalyzed reactions and overlocked the economic viability. This review critically discusses the factors affecting the promotion of the economic feasibility of the enzymatic processes from the lab to large scale. These include reactor configuration, type of feedstock, conditions optimization, immobilization, lipase-producing microorganisms, and substrate diversification. In addition, this review reports the recent advances in lipase-catalyzed production of fatty acids, fatty esters, monoglycerides, and biodiesel in the lab as well as in the large-scales. To the best of authors' knowledge, this is the first review article reports the recent global progress achieved in both lab-and large-scale for the enzymatic production of OC/BD.

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