Histologic and proteomic profile of two methods to decellularize human dental pulp tissue

Objective: The present study compared the structural and proteomic architecture of extracellular matrices (ECM) of decellularized human dental pulp using two previously described protocols.Design: Pulp tissue from 150 molars was extracted and three treatments took place, based on the MatougElwerfelli Group (MG) and the Song Group (SG) protocols and an untreated pulp group (CG), to examine histoarchitecture and the effectiveness of the decellularization process, using histological analysis (n = 12) and scanning electron microscopy (SEM) (n = 3). Protein extraction took place using 100 mg dry weight of pulp, in triplicates for each group, and the shotgun proteome analysis was performed by nanoUPLC-MSE. Proteins were identified using the revised human UNIPROT database attached to the PLGS search engine.Results: Histological analysis and SEM demonstrated that ECM in MG was more preserved. Proteome analysis showed that the decellularized process in MG maintained approximately 69.56% of proteins identified in untreated pulp tissue while SG maintained 28.26%.Conclusions: ECM appears to be suitable as a potential biological scaffold for pulp revascularization and regeneration procedures, especially those processed according to the Matoug-Elwerfelli protocol. This finding can collaborate to enhance clinical solutions for young permanent teeth that have suffered necrosis.

Automated summary

This study compared the structural and proteomic architecture of decellularized human dental pulp using two different protocols. The results showed that the ECM preserved in the MG protocol was more intact. Proteomic analysis revealed that the MG protocol maintained around 69.56% of the proteins identified in the untreated pulp tissue, while the SG protocol only maintained 28.26%.