Glucuronan lyases from family PL7 use a Tyr/Tyr syn β-elimination catalytic mechanism for glucuronan breakdown

Review Biochemistry & Molecular Biology

Molecular mechanisms of processive glycoside hydrolases underline catalytic pragmatism

Maria Hrmova et al.

Summary: This article discusses the difference between processive and distributive catalysis in enzyme conversion. It focuses on the processive catalysis in glycoside hydrolases, particularly the substrate-product-assisted processive catalysis in the GH3 family enzymes. The study of GH3 enzymes provides valuable insights for theoretical and practical applications in bioengineering.

BIOCHEMICAL SOCIETY TRANSACTIONS (2023)

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Article Biotechnology & Applied Microbiology

Discovery of a Novel Glucuronan Lyase System in Trichoderma parareesei

Bo Pilgaard et al.

Summary: This study reports the discovery of a set of six complementary glucuronan lyase enzymes in the mycoparasite Trichoderma parareseei. The major finding of this study is that the majority of these lyases are induced when T. parareesei is inoculated on Basidiomycete cell walls that contain glucuronan. The study also reveals putative glucuronan lyase encoding genes in a wealth of other fungi, suggesting that fungal cell wall glucuronan is a target C-source for many types of fungi. The findings may have important implications for controlled production of glucuronan oligomers for advanced pharmaceutical applications and the development of new fungal biocontrol agents.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2022)

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Article Biochemistry & Molecular Biology

The carbohydrate-active enzyme database: functions and literature

Elodie Drula et al.

Summary: The CAZy database, freely available for browsing and download, is deeply rooted in human curation and plays a crucial role in maintaining and updating family classification, classifying new sequences, and presenting functional information. Over the past 8 years, there has been an increase in novel families and extensive annotations conducted, highlighting the significant amount of work involved in analyzing and reporting biochemical data from the literature.

NUCLEIC ACIDS RESEARCH (2022)

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Article Chemistry, Physical

Model of Processive Catalysis with Site Clustering and Blocking and Its Application to Cellulose Hydrolysis

Zdenek Petrasek et al.

Summary: Interactions between particles moving on a linear track and obstacles can lead to crowding, hindering transport kinetics. Cellulose hydrolysis by enzymes on cellulose chains is an example. The exact role and mechanism of crowding and blocking in cellulose hydrolysis are not clear. This study proposes a simple model that incorporates crowding and blocking effects by distinguishing between binding and non-binding sites on the chain. The model reproduces experimental results related to the early phase of cellulose hydrolysis and suggests periodic desorption and rebinding of enzyme molecules as a strategy to enhance the rate of cellulose hydrolysis.

JOURNAL OF PHYSICAL CHEMISTRY B (2022)

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Article Chemistry, Multidisciplinary