Toxicity of binary-metal mixtures (As, Cd, Cu, Fe, Hg, Pb and Zn) in the euryhaline rotifer Proales similis: Antagonistic and synergistic effects

Data regarding the effects of binary metal mixtures in marine zooplankton are scarce, particularly for rotifers. We examined the toxicity of 21 binary equitoxic mixtures of As, Cd, Cu, Fe, Hg, Pb, and Zn on the euryhaline rotifer Proales similis. The toxic units (TU50) revealed that 20 of these binary mixtures exhibited synergistic effects (TU50 < 1.00). The As-Hg mixture showed a strong antagonistic effect (TU50 = 2.39), whereas the Hg-Cu interaction exhibited a significant synergistic effect (TU50 = 0.29) on P. similis. TU50 values were <0.60 in all cases that showed synergism (80 %). Regarding the MIXTOX analysis, 13 binary mixtures presented some level of synergism, while two mixtures presented only additivity. Results emphasize the need for environmental agencies to revise and readjust protection guidelines for marine biota in response to the evident synergistic effects occurring at metal mixtures concentrations below the current permissible limits.

Automated summary

Data is lacking on the effects of binary metal mixtures on marine zooplankton, especially rotifers. This study examined the toxicity of 21 equitoxic binary mixtures of As, Cd, Cu, Fe, Hg, Pb, and Zn on the euryhaline rotifer Proales similis. The results showed that the majority of these mixtures exhibited synergistic effects, highlighting the need for revising protection guidelines for marine biota.