期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 401, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2023.135005
关键词
Label-free; Single-base mutation; Plasmonic sensor; Surface-enhanced Raman spectroscopy; Clinical diagnosis
Surface-enhanced Raman spectroscopy (SERS) has great potential in DNA analysis, but the identification of single-base mutations is still limited. In this work, plasmonic SiNW arrays decorated with Ag nanoparticles (SiNW@AgNP arrays) were developed to address these challenges, showing excellent sensitivity and reproducibility. Under optimized conditions, SiNW@AgNP arrays enable the detection of DNA bases and the discrimination of gene mutations.
Surface-enhanced Raman spectroscopy (SERS) has exhibited great potential in deoxyribonucleic acid (DNA) analysis. Rapid and label-free identification of single-base mutation in DNA is still limited by the sensitivity and reproducibility of SERS substrates. In this work, we develope plasmonic SiNW arrays decorated with Ag nano particles (SiNW@AgNP arrays) via hexadecyltrimethylammonium bromide (CTAB)-assisted galvanic reaction to address these challenges, which show excellent sensitivity and reproducibility. Under optimized experimental conditions, plasmonic SiNW@AgNP arrays enable detection of DNA bases of adenine (A), guanine (G), cytosine (C), and thymine (T) in solution at concentration down to pM-mu M range, and multiple detection ability with mixture of DNA bases. More significantly, our study demonstrates the SiNW@AgNP arrays may serve as excellent SERS substrates in discriminating DNA single-base mutation between sickle cell anemia gene and normal hemoglobin gene in serum samples, which can be exploited in a wide range of plasmonic enhanced sensing applications for a fast, real-time, label-free and cost-effective clinical diagnosis.
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