期刊
REGENERATIVE THERAPY
卷 25, 期 -, 页码 24-34出版社
ELSEVIER
DOI: 10.1016/j.reth.2023.11.008
关键词
Polyethylene glycol; Injectable hydrogel; Gel -gel phase separation; Growth factors; Sustained release; Bone regeneration
In this study, we evaluated the potential of oligo-tetra-PEG gel (Oligo gel) as a growth factor-releasing scaffold. Compared to tetra-PEG hydrogel (Tetra gel), Oligo gel underwent a higher degree of gel-gel phase separation, demonstrating better protein release and osteogenic potential, and achieving superior regeneration in a mouse calvarial defect model. Therefore, Oligo gel is considered a promising therapeutic biomaterial.
With the rising prevalence of bone-related injuries, it is crucial to improve treatments for fractures and defects. Tissue engineering offers a promising solution in the form of injectable hydrogel scaffolds that can sustain the release of growth factors like bone morphogenetic protein-2 (BMP-2) for bone repair. Recently, we discovered that tetra-PEG hydrogels (Tetra gels) undergo gel-gel phase separation (GGPS) at low polymer content, resulting in hydrophobicity and tissue affinity. In this work, we examined the potential of a newer class of gel, the oligo-tetra-PEG gel (Oligo gel), as a growth factor-releasing scaffold. We investigated the extent of GGPS occurring in the two gels and assessed their ability to sustain BMP-2 release and osteogenic potential in a mouse calvarial defect model. The Oligo gel underwent a greater degree of GGPS than the Tetra gel, exhibiting higher turbidity, hydrophobicity, and pore formation. The Oligo gel demonstrated sustained protein or growth factor release over a 21-day period from protein release kinetics and osteogenic cell differentiation studies. Finally, BMP-2-loaded Oligo gels achieved complete regeneration of critical-sized calvarial defects within 28 days, significantly outperforming Tetra gels. The easy formulation, injectability, and capacity for sustained release makes the Oligo gel a promising candidate therapeutic biomaterial. (c) 2023, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/ 4.0/).
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