期刊
ZOOLOGICAL RESEARCH
卷 44, 期 6, 页码 1080-1094出版社
SCIENCE PRESS
DOI: 10.24272/j.issn.2095-8137.2023.317
关键词
Tree shrews; Spermatogonial stem cells; Nicotinamide riboside; DNA damage; Mitochondrial dysfunction
类别
In this study, the in vivo spermatogenesis potential of tree shrew SSCs cultured across different passages was examined. It was found that long-term expansion (>50 passages) led to the loss of spermatogenesis ability in SSCs. RNA sequencing analysis revealed significant gene expression changes after sub-culturing primary SPG lines for more than 40 passages, and experimental validation confirmed DNA damage accumulation and mitochondrial dysfunction in high-passage cells. Supplementation with NAD(+) precursor NR showed beneficial effects in reducing DNA damage accumulation and mitochondrial dysfunction. This research provides a comprehensive analysis of the genetic and physiological attributes critical for the sustained expansion of undifferentiated SSCs in tree shrews and proposes an effective strategy for extended in vitro maintenance.
Tree shrews ( Tupaia belangeri chinensis) share a close relationship to primates and have been widely used in biomedical research. We previously established a spermatogonial stem cell (SSC)-based gene editing platform to generate transgenic tree shrews. However, the influences of long-term expansion on tree shrew SSC spermatogenesis potential remain unclear. Here, we examined the in vivo spermatogenesis potential of tree shrew SSCs cultured across different passages. We found that SSCs lost spermatogenesis ability after long-term expansion (>50 passages), as indicated by the failure to colonize the seminiferous epithelium and generate donor spermatogonia (SPG)-derived spermatocytes or spermatids marking spermatogenesis. RNA sequencing (RNA-seq) analysis of undifferentiated SPGs across different passages revealed significant gene expression changes after sub-culturing primary SPG lines for more than 40 passages on feeder layers. Specifically, DNA damage response and repair genes (e.g., MRE11, SMC3, BLM, and GEN1) were down-regulated, whereas genes associated with mitochondrial function (e.g., NDUFA9, NDUFA8, NDUFA13, and NDUFB8) were up-regulated after expansion. The DNA damage accumulation and mitochondrial dysfunction were experimentally validated in high-passage cells. Supplementation with nicotinamide adenine dinucleotide (NAD (+)) precursor nicotinamide riboside (NR) exhibited beneficial effects by reducing DNA damage accumulation and mitochondrial dysfunction in SPG elicited by long-term culture. Our research presents a comprehensive analysis of the genetic and physiological attributes critical for the sustained expansion of undifferentiated SSCs in tree shrews and proposes an effective strategy for extended in vitro maintenance.
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