A dynamic Eu(III)-macrocycle served as the turn-on fluorescent probe for distinguishing H2O from D2O

H2O and D2O are an important pair of analogues, and their high-efficient detections are closely related to fields of chemical industry, food processing, semiconductor, environmental monitoring, etc. Because of their extremely similar physical and chemical properties, H2O and D2O can be mutually soluble in any ratios, and it is generally thought that the discrimination of H2O and D2O is an enormous challenge. Herein, upon the fact that vibrational frequency of O-H is greater than O- D, we design a dynamic Eu(III)-macrocycle Eu-2a with two emitters which exhibits the imine bond breakage of macrocycle emitter H2L2a in H2O or D2O, resulting in the turn-on fluorescence of Eu(III) emitter. For their differential fluorescence sensing signals of Eu-2a on three emission bands (433, 500 and 615 nm), the statistical analysis method is employed to produce fully separated fingerprints and thus high-throughput discrimination of 13 common solvents, especially the H2O and D2O. Fluorescent titration experiments by instrumental or smartphone-based analysis method also prove the successful determination of proportional H2O/D2O mixtures together with the good sensitivity and wide linear response range. Moreover, this H2O-triggered fluorescent complex Eu-2a used as the fluorescence ink also shows its potential in information encryption application. This article must be a valuable reference for the areas of lanthanide-based luminescent material, multianalyte detection and information encryption.

Automated summary

H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.