The Effect of Banana Rhizosphere Chemotaxis and Chemoattractants on Bacillus velezensis LG14-3 Root Colonization and Suppression of Banana Fusarium Wilt Disease

Fusarium oxysporum f. sp. cubense (Foc) causes banana Fusarium wilt disease, which is a destructive soil-borne disease. Many plants can recruit rhizosphere microorganisms using their root exudates, thereby shaping the rhizosphere microbiome to resist pathogen infection. Therefore, this study was conducted to explore the role of root exudates in the process of biocontrol strain colonization and resistance to pathogens. In this study, the banana root exudates used as chemoattractants were obtained by hydroponics. Bacillus velezensis strain LG14-3 was isolated from the infected area of the root system of banana and showed significant chemotaxis to banana root exudates and strong inhibition of Fusarium oxysporum f. sp. cubense. Further analysis found that LG14-3 showed chemotaxis toward the components of banana root exudates, such as citric acid, succinic acid, glycine, D-galactose and D-maltose, and glycine and citric acid, which resulted in more significant chemotaxis of LG14-3. Moreover, banana root exudates enhanced the swarming motility and biofilm formation of LG14-3. Pot experiments showed that glycine and citric acid enhanced the colonization ability of Bacillus velezensis LG14-3 in the banana rhizosphere and reduced the disease severity index of banana fusarium wilt. Glycine and citric acid enhanced the growth-promoting ability of LG14-3 under pathogen stress. Our results showed that the addition of chemotactic substances enhanced the biocontrol potential of Bacillus velezensis LG14-3 to prevent banana Fusarium wilt.

Automated summary

This study explored the role of banana root exudates in the colonization of biocontrol strain Bacillus velezensis LG14-3 and its resistance to Fusarium oxysporum f. sp. cubense. The root exudates attracted LG14-3 and enhanced its colonization ability in the banana rhizosphere. Additionally, the root exudates increased LG14-3's swarming motility and biofilm formation. Pot experiments showed that glycine and citric acid enhanced LG14-3's colonization ability and reduced the severity of banana fusarium wilt.