期刊
CELLULAR & MOLECULAR IMMUNOLOGY
卷 20, 期 3, 页码 277-291出版社
CHIN SOCIETY IMMUNOLOGY
DOI: 10.1038/s41423-022-00972-0
关键词
RNA helicases; B cell activation; Germinal center reaction
类别
Using CRISPR/Cas9-mediated functional analysis and mouse genetics approaches, this study found that the activation-induced RNA helicase Dhx33 plays a critical role in coupling B-cell activation with growth and proliferation. Dhx33-deficient B cells exhibited impaired B-cell development, germinal center reactions, plasma cell differentiation, and antibody production.
Upon recognition of foreign antigens, naive B cells undergo rapid activation, growth, and proliferation. How B-cell growth and proliferation are coupled with activation remains poorly understood. Combining CRISPR/Cas9-mediated functional analysis and mouse genetics approaches, we found that Dhx33, an activation-induced RNA helicase, plays a critical role in coupling B-cell activation with growth and proliferation. Mutant mice with B-cell-specific deletion of Dhx33 exhibited impaired B-cell development, germinal center reactions, plasma cell differentiation, and antibody production. Dhx33-deficient B cells appeared normal in the steady state and early stage of activation but were retarded in growth and proliferation. Mechanistically, Dhx33 played an indispensable role in activation-induced upregulation of ribosomal DNA (rDNA) transcription. In the absence of Dhx33, activated B cells were compromised in their ability to ramp up 47S ribosomal RNA (rRNA) production and ribosome biogenesis, resulting in nucleolar stress, p53 accumulation, and cellular death. Our findings demonstrate an essential role for Dhx33 in coupling B-cell activation with growth and proliferation and suggest that Dhx33 inhibition is a potential therapy for lymphoma and antibody-mediated autoimmune diseases.
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