期刊
BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
卷 16, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13068-022-02237-4
关键词
Biotransformations; Cyanobacteria; d-Glucose; Synechocystis sp; PCC 6803; Ene-reduction
It has been found that the addition of exogenous carbon source D-glucose can accelerate NAD(P)H redox reactions and increase the activity of ene-reductase YqjM in Synechocystis sp. PCC 6803, leading to higher production of biofuels and catalysis in heterologous electron consuming processes.
Background Cyanobacteria have emerged as highly efficient organisms for the production of chemicals and biofuels. Yet, the productivity of the cell has been low for commercial application. Cyanobacterial photobiotransformations utilize photosynthetic electrons to form reducing equivalents, such as NADPH-to-fuel biocatalytic reactions. These photobiotransformations are a measure to which extent photosynthetic electrons can be deviated toward heterologous biotechnological processes, such as the production of biofuels. By expressing oxidoreductases, such as YqjM from Bacillus subtilis in Synechocystis sp. PCC 6803, a high specific activity was obtained in the reduction of maleimides. Here, we investigated the possibility to accelerate the NAD(P)H-consuming redox reactions by addition of carbohydrates as exogenous carbon sources such as D-Glucose under light and darkness.Results A 1.7-fold increase of activity (150 mu mol min(-1) gDCW(-1)) was observed upon addition of D-Glucose at an OD750=2.5 (DCW=0.6 g L-1) in the biotransformation of 2-methylmaleimide. The stimulating effect of D-Glucose was also observed at higher cell densities in light and dark conditions as well as in the reduction of other substrates. No increase in both effective photosynthetic yields of Photosystem II and Photosystem I was found upon D-Glucose addition. However, we observed higher NAD(P)H fluorescence when D-Glucose was supplemented, suggesting increased glycolytic activity. Moreover, the system was scaled-up (working volume of 200 mL) in an internally illumi-nated Bubble Column Reactor exhibiting a 2.4-fold increase of specific activity under light-limited conditions.Conclusions Results show that under photoautotrophic conditions at a specific activity of 90 mu mol min(-1) gDCW(-1), the ene-reductase YqjM in Synechocystis sp. PCC 6803 is not NAD(P)H saturated, which is an indicator that an increase of the rates of heterologous electron consuming processes for catalysis and biofuel production will require funnelling further reducing power from the photosynthetic chain toward heterologous processes.
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