Vending-Machine-Style Skin Excretion Sensing

Skin metabolites show huge potential for use in clinical diagnostics. However, skin sampling and analysis workflows are tedious and time-consuming. Here, we demonstrate a vending-machine-style skin excretion sensing platform based on hydrogel-assisted sampling of skin metabolites. In this sensing platform, a sampling probe with hydrogel is held by a robotic arm. The robotic arm manoeuvres the probe to press it onto the forearm of a human subject. Due to the highly hydrophilic nature of the hydrogel, water-soluble metabolites -released by skin -are collected into the hydrogel, leaving behind the nonpolar metabolites. The probe is then inserted into a custom-made open port sampling interface coupled to an electrospray ion source of a high-resolution quadrupole-time-of-flight mass spectrometer. Metabolites in the hydrogel are immediately extracted by a solvent liquid junction in the interface and analyzed using the mass spectrometer. The ion current of the target analyte is displayed on a customized graphical user interface, which can also be used to control the key components of the analytical platform. The automated sampling and analysis workflow starts after the user inserts coins or presents an insurance card, presses a button, and extends an arm on the sampling area. The platform relies on low-cost mechanical and electronic modules (a robotic arm, a single board computer, and two microcontroller boards). The limits of detection for standard analytes-arginine, citrulline, and histidine- embedded in agarose gel beds were 148, 205, and 199 nM, respectively. Various low-molecular-weight metabolites from human skin have been identified with the high-resolution mass spectrometer.

Automated summary

Skin metabolites have promising potential for clinical diagnostics, but current sampling and analysis methods are time-consuming. In this study, researchers developed a vending-machine-style platform that uses hydrogel-assisted sampling to collect skin metabolites. The platform involves a robotic arm holding a sampling probe with hydrogel, which is pressed onto the forearm to capture water-soluble metabolites. The probe is then analyzed using a high-resolution mass spectrometer, with results displayed on a user interface.