Changes in Alternative Splicing Revealed Special Metabolic Pathways Related to Heterosis of Heading Chinese Cabbage

As an important genetic improvement technique in current production practice, heterosis is widely used to enhance the productive traits of hybrid progeny from their parents. Alternative splicing (AS) analysis can be used as a method for exploring the molecular manifestations of heterosis. In our research, 16 hybrids and their parents were utilized to analyze the heterosis performance and AS events. Statistics of plant gross weight (PGW) showed that these hybrids had prominent heterosis, with the mid-parent heterosis values (MPV) ranging from 15.69% to 233.98%. Through pairwise comparison among the female parent, male parent, and hybrid, there were 2980-3205 AS events in each combination, with intron retention being the most common type followed by alternate 3' splice site, alternative 5' splice site, skipped exon, and mutually exclusive exon.There were 263-409 differential AS genes (DASGs) between the female parent and the hybrid, and 234-425 DASGs between the male parent and the hybrid in cross combinations. The DASGs were significantly enriched in 33 metabolic pathways in 16 cross combinations, and DASGs of different cross combinations were enriched in different metabolic pathways. Moreover, 76 DASGs in the strong heterosis combinations were identified and significantly enriched in the metabolic pathways related to amino acid metabolism. Further analysis revealed that most of these DASGs in amino acid metabolism were expressed differently in strong heterosis combinations. In addition, the expression levels of BraA06g014310.3C and BraA03g041700.3C in amino acid metabolism significantly correlated with PGW. These results could provide an index for future studies of the genetic and molecular mechanism of heterosis in hybrids.

Automated summary

Heterosis, an important genetic improvement technique, is widely used to enhance the productive traits of hybrid progeny. In this study, heterosis performance and alternative splicing (AS) events were analyzed in 16 hybrids and their parents. Significant heterosis was observed in these hybrids, with a wide range of mid-parent heterosis values (MPV). There were various AS events in each combination, with intron retention being the most common type. Differential AS genes (DASGs) were identified between the parents and the hybrids, and were significantly enriched in metabolic pathways. DASGs in amino acid metabolism were found to be associated with strong heterosis combinations and plant gross weight (PGW).