Shade-Induced Effects on Essential Oil Yield, Chemical Profiling, and Biological Activity in Some Lamiaceae Plants Cultivated in Serbia

Thyme, mint, and lemon balm were used to determine whether shading conditions could improve the yield, composition, antioxidant, and antimicrobial activity in plant essential oils (EOs) in comparison with non-shaded plants from an open field. The yield of the EOs of non-shaded thyme, mint, and lemon balm, was 3.44, 3.96, and 0.21 mL/100 g, respectively. Plants covered by nets produced different levels of EOs (3.46, 2.20, and 0.45 mL/100 g) after 120 min of hydrodistillation. The main components of the thyme essential oil are thymol (44.2-43.9%), gamma-terpinene (18.3-16.8%), and p-cymene (16.5-17.4%). The predominant components of mint essential oil are piperitenone oxide (52.6-64.8%) and 1,8 cineole (25.9-16.3%), while lemon balm essential oil consists of the following main components: geranial (34.0-32.8%); neral (21.3-24.9%); and piperitenone oxide (17.2-16.7%). The EOs from non-shaded thyme and mint plants have the highest antioxidant activity (EC50 value 0.54 mg/mL and 3.03 mg/mL). However, shaded lemon balm showed a stronger antioxidant activity (EC50 3.43 mg/mL) than non-shaded plants (12.85 mg/mL) after 60 min of incubation. The EOs from all plants showed significant effects against Escherichia coli, Proteus vulgaris, Bacillus subtilis, Staphylococcus aureus, and Candida albicans. The most active EOs against most of the isolates originated from Thymus vulgaris L., plants. Adequate cultivation techniques, such as shading for Lamiaceae plants, has positive effects, especially in Melissa officinalis L. Shading can achieve a higher content and components in terms of the specific biological activity (antioxidant and microbial) of EOs.

Automated summary

This study showed that shading conditions could improve the yield, composition, antioxidant, and antimicrobial activity of essential oils (EOs) from thyme, mint, and lemon balm plants. Thyme and mint EOs from non-shaded plants had the highest antioxidant activity, while shaded lemon balm showed stronger antioxidant activity than non-shaded plants. The EOs from all plants exhibited significant effects against several microbial strains. Proper cultivation techniques, such as shading for Lamiaceae plants, can enhance the biological activity of EOs.