Mitigating the nephrotoxic impact of hexavalent chromium in Ctenopharyngodon idellus (grass carp) with Boerhavia diffusa (punarnava) leaf extract

In Ctenopharyngodon idellus, the ameliorative influence of rutin-containing leaf extract of Boerhavia diffusa was assessed against chronic exposure to hexavalent chromium. For this, alterations in chromium accumulation, oxidative stress, kidney function markers, histopathology (light and transmission electron microscopy), and transcriptional profiling (Nrf2 and MT2) were examined. RP-HPLC analysis confirmed the presence of rutin (90.45 +/- 0.98 mg/g) in the ethanolic leaf extract of the plant. LD50 of the extract to the fish was beyond 5000 mg/kg b.w. The fish was subjected to a sublethal concentration of hexavalent chromium (5.30 mg/L) accompanied by a dose of 250 mg/kg b.w./day of extract in the diet for the experimental duration of 45 days. The extract alone did not generate any adverse consequences in the nephric tissue. Chronic exposure to hexavalent chromium damaged tissue irreparably, demonstrated by elevated levels of kidney function markers (blood urea nitrogen and creatinine) and altered histoarchitecture (DTC value of 78.02 +/- 10.5). The metal exposure increased chromium accumulation and malondialdehyde (MDA) and decreased the reduced glutathione (GSH) levels, the activity of antioxidant enzymes (superoxide dismutase, catalase and glutathione-S-transferase) and gene expression in the tissue. The co-supplementation of leaf extract with metal exposure revealed a tissue architecture with normal to slight modifications, and the level of kidney markers, antioxidants, and genes expressed in a normalized range. Principal component analysis created two components with antioxidants (GSH, SOD, CAT, and GST) revealing a negative correlation with the second component comprising MDA, DTC, and chromium concentration. It can be concluded that B. diffusa leaves are safe additives in the fish diet and possess an ameliorative capacity for renal injury incurred by hexavalent chromium.

Automated summary

This study assessed the ameliorative effects of Boerhavia diffusa leaf extract on chronic exposure to hexavalent chromium in Ctenopharyngodon idellus. The results showed that the extract reduced kidney damage caused by hexavalent chromium and regulated oxidative stress, kidney function markers, histopathology, and gene expression. This suggests that Boerhavia diffusa leaf extract has a protective effect against renal injury.