4.5 Review

Technological advances in three-dimensional skin tissue engineering

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3D bioprinting of an implantable xeno-free vascularized human skin graft

Tania Baltazar et al.

Summary: This study successfully isolated, expanded, and cryopreserved human endothelial cells, fibroblasts, pericytes, and keratinocytes under xeno-free conditions. A bioprinted vascularized bilayered skin substitute was fabricated using xeno-free cultured cells, and after transplantation, the cells formed a mature stratified epidermis and perfused microvessels. This study demonstrates the feasibility of a xeno-free approach to complex tissue engineering.

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Biobridge: An Outlook on Translational Bioinks for 3D Bioprinting

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Summary: 3D bioprinting has the potential to overcome deficiencies of traditional tissue engineering by developing bioinks with clinical applicability, enabling the printing of stable structures that evolve into functional tissue in vivo. A biology-based approach is advocated for engineering bioinks, emphasizing efficiency and reproducibility for clinical translation.

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Article Pharmacology & Pharmacy

The 3D Bioprinted Scaffolds for Wound Healing

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Summary: This review discusses the latest developments in skin tissue engineering using 3D bioprinting as a new tool. It provides an overview of current bioprinting methods and summarizes bioink formulations, parameters, and properties. Representative examples and advances in the field, as well as limitations and future needs, are also discussed.

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Article Engineering, Biomedical

Tailoring bioinks of extrusion-based bioprinting for cutaneous wound healing

Yuzhen Wang et al.

Summary: This review discusses the potential and limitations of extrusion-based bioprinting (EBB) for skin regeneration, and introduces strategies for improving the physical properties of bioinks and reinforcing bioinks in EBB approaches. It highlights the applications and effects of current EBB-based bioinks on wound healing, wound scar formation, vascularization, and the regeneration of skin appendages, along with the challenges and future perspectives.

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3D Bioprinting Constructs to Facilitate Skin Regeneration

Luciana Y. Daikuara et al.

Summary: This review summarizes the application of 3D bioprinting technology in skin regeneration in recent years, covering aspects such as bioink formulations, cell combinations, and manufacturing methods, while also addressing issues involving the selection of suitable materials and cells to ensure the functionality of skin constructs.

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Mechanical properties of polycaprolactone (PCL) scaffolds for hybrid 3D-bioprinting with alginate-gelatin hydrogel

Fritz Koch et al.

Summary: The generation of artificial human tissue using 3D bioprinting has become an important research topic in recent years. In this study, the authors evaluate the process parameters relevant to a hybrid bioprinting process, involving the printing of thermoplastic material and a cell-laden hydrogel. Through their experiments, they determine the optimal printing temperature and filament bonding strength for successful tissue generation. They also examine the mechanical strength of the printed structures and observe that scaffolds made using the hybrid bioprinting technique show promising mechanical properties.

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In Situ Crosslinkable Collagen-Based Hydrogels for 3D Printing of Dermis-Mimetic Constructs

Moon Sung Kang et al.

Summary: Researchers have developed tyramine-conjugated hyaluronic acid and collagen hydrogel bioinks for the 3D printing of dermis-mimetic constructs. The hydrogel exhibits favorable physicochemical properties and 3D printability, and provides a cytocompatible microenvironment.

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Article Engineering, Biomedical

Functionalizing multi-component bioink with platelet-rich plasma for customized in-situ bilayer bioprinting for wound healing

Ming Zhao et al.

Summary: This study prepared PRP-integrated AG composite hydrogel bioinks and demonstrated its positive biological effects, including improved cellular behavior, enhanced angiogenesis, and accelerated wound closure. This study provides a theoretical basis for the use of PRP in rapid individualized wound repair.

MATERIALS TODAY BIO (2022)

Article Chemistry, Multidisciplinary

Alginate-Lysozyme Nanofibers Hydrogels with Improved Rheological Behavior, Printability and Biological Properties for 3D Bioprinting Applications

Maria C. Teixeira et al.

Summary: In this study, alginate nanocomposite hydrogel bioinks reinforced with lysozyme nanofibers were developed. The bioinks showed improved rheological performance, printability, and biological properties, making them a promising solution to overcome the limitations of alginate-based bioinks. The optimized A-LNF inks exhibited good shear-thinning behavior, recovery properties, and noncytotoxicity, providing a solid foundation for bioprinting applications.

NANOMATERIALS (2022)

Review Chemistry, Multidisciplinary

Research progress of carbon materials in the field of three-dimensional printing polymer nanocomposites

Li Bianhong et al.

Summary: This article introduces the principles, characteristics, and research progress of 3D printing technology, focusing on the application of carbon nanomaterial-reinforced polymer composites. It also analyzes the commercial applications of 3D printing in this field.

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A State-of-the-Art Review of Laser-Assisted Bioprinting and its Future Research Trends

Chaoran Dou et al.

Summary: Bioprinting technology, specifically laser-assisted bioprinting, offers high resolution and cell viability for precise cell control in reconstructing living organs. Recent studies highlight the developments, advantages, disadvantages, potential applications, technical challenges, and future research trends of LAB technologies.

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Investigation of rheology, printability, and biocompatibility of N,O-carboxymethyl chitosan and agarose bioinks for 3D bioprinting of neuron cells

Haley M. Butler et al.

Summary: This study found that a balanced mixture of 40% agarose and 60% NOOC is the most suitable bioink for 3D bioprinting, ensuring both cell viability and printability.

MATERIALIA (2021)

Article Biophysics

3D bioprinting for fabricating artificial skin tissue

Chuang Gao et al.

Summary: The skin, as the first line of defense, is susceptible to damage and there is a growing demand for artificial skin in various fields. Traditional skin tissue engineering has made progress, but there are challenges and prospects in 3D bioprinting.

COLLOIDS AND SURFACES B-BIOINTERFACES (2021)

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Bioengineered Skin Substitutes: Advances and Future Trends

Shima Tavakoli et al.

Summary: The skin, as the largest organ in the human body, plays a crucial role in maintaining balance and protection from external factors. The use of human bioengineered skin substitutes offers an alternative approach to treat large skin defects caused by burns or trauma, with different types available based on chemical composition and skin component. Advances in three-dimensional bioprinting techniques have also contributed to the development of skin substitutes for optimal wound healing.

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Engineering of diseased human skin equivalent using 3D cell printing for representing pathophysiological hallmarks of type 2 diabetes in vitro

Byoung Soo Kim et al.

Summary: The study successfully developed a 3D diseased skin tissue model with pathophysiological hallmarks of type 2 diabetes using 3D cell printing technique. By incorporating a perfusable vascularized diabetic hypodermis, the structural similarities and diabetic properties of the model were enhanced. The feasibility of using this new disease model for drug development was demonstrated through the application of test drugs.

BIOMATERIALS (2021)

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Current Insight of Printability Quality Improvement Strategies in Natural-Based Bioinks for Skin Regeneration and Wound Healing

Syafira Masri et al.

Summary: The article introduces the application of skin tissue engineering and the latest research progress using 3D bioprinting technology to manufacture scaffolds, and discusses the limitations of current bioinks as well as the impact of the optimal printing technology application temperature.

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Investigations of Graphene and Nitrogen-Doped Graphene Enhanced Polycaprolactone 3D Scaffolds for Bone Tissue Engineering

Weiguang Wang et al.

Summary: Research on high-performance nitrogen-doped graphene as a functional filler for PCL scaffolds to develop the next generation of electro-active scaffolds shows improved in vitro biological performance.

NANOMATERIALS (2021)

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3D Bioprinting of Functional Skin Substitutes: From Current Achievements to Future Goals

Paula Gabriela Manita et al.

Summary: This review discusses 3D bioprinting of skin substitutes as an efficient approach for managing skin injuries. It explains the basic principles of bioprinting, popular methods and biomaterial choices for 3D-printed skin construct production, and the advantages over other production methods. The chronological development of this technology and the need for multidisciplinary efforts to optimize designs, biomaterials and production processes are highlighted.

PHARMACEUTICALS (2021)

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Diving into 3D (bio)printing: A revolutionary tool to customize the production of drug and cell-based systems for skin delivery

Sara Bom et al.

Summary: The incorporation of 3D printing technologies in the pharmaceutical industry can revolutionize the way drugs are developed, especially in the customization of dosages and the creation of localized drug delivery systems. This high-resolution technology can reduce production costs and improve patient compliance, but potential drawbacks include skin irritation, sensitization, and high costs.

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3D Bioprinted Skin Substitutes for Accelerated Wound Healing and Reduced Scar

Qin Lian et al.

Summary: Bionically designed bilayer skin fabricated using 3D bioprinting showed promising results in accelerating wound healing and reducing scarring in large skin injuries.

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Hydrodynamic Focusing-Enabled Blood Vessel Fabrication for in Vitro Modeling of Neural Surrogates

Kyle A. DiVito et al.

Summary: This study demonstrates the use of hydrodynamic focusing to fabricate blood vessels consisting of primary human endothelial cells and normal human primary astrocytes. The mature microvessels exhibit key features and can be used to study vascular dynamics and the blood-brain barrier.

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Progress of 3D Bioprinting in Organ Manufacturing

Dabin Song et al.

Summary: Three-dimensional bioprinting technology can assemble biomaterials including cells and bioactive agents layer by layer, successfully printing living tissues and organs, significantly improving organ manufacturing capabilities.

POLYMERS (2021)

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Natural and Synthetic Bioinks for 3D Bioprinting

Roghayeh Khoeini et al.

Summary: Bioprinting technology offers precise control over the geometric and compositional attributes of tissue constructs, using an automated approach to fabricate functional and mechanically stable structures. Bioinks, consisting of hydrogel materials and living cells, play a critical role in encapsulating appropriate cells to create functional tissue structures.

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3D bioprinting for skin tissue engineering: Current status and perspectives

Tingting Weng et al.

Summary: 3D bioprinting is a rapidly reliable method for skin regeneration, with the ability to customize skin shape and high resolution, flexibility, and reproducibility. It shows great potential in the fabrication of tissue-engineered skin.

JOURNAL OF TISSUE ENGINEERING (2021)

Review Materials Science, Biomaterials

Recent progress in extrusion 3D bioprinting of hydrogel biomaterials for tissue regeneration: a comprehensive review with focus on advanced fabrication techniques

Mohsen Askari et al.

Summary: This study summarizes the recent advances in 3D bioprinting for functional tissue development over the past decade, focusing on key parameters such as bioink selection, printing conditions, and tissue scaffold design. It highlights both challenges and opportunities, as well as the latest bioprinted scaffold technologies.

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