Lactobacillus rhamnosus GG Promotes Recovery of the Colon Barrier in Septic Mice through Accelerating ISCs Regeneration

Disruption of the intestinal barrier is both the cause and result of sepsis. The proliferation and differentiation of intestinal stem cells (ISCs) promote the regenerative nature of intestinal epithelial cells, repairing the injured intestinal mucosal barrier; however, it is uncertain whether the recovery effects mediated by the ISCs are related to the gut microbiota. This research found that the survival rate of septic mice was improved with a Lactobacillus rhamnosus GG (LGG) treatment. Furthermore, an increased proliferation and decreased apoptosis in colon epithelial cells were observed in the LGG-treated septic mice. In vitro, we found that a LGG supernatant was effective in maintaining the colonoid morphology and proliferation under the damage of TNF-alpha. Both in the mice colon and the colonoid, the LGG-induced barrier repair process was accompanied by an increased expression of Lgr5(+) and lysozyme(+) cells. This may be attributed to the upregulation of the IL-17, retinol metabolism, NF-kappa B and the MAPK signaling pathways, among which, Tnfaip3 and Nfkbia could be used as two potential biomarkers for LGG in intestinal inflammation therapy. In conclusion, our finding suggests that LGG protects a sepsis-injured intestinal barrier by promoting ISCs regeneration, highlighting the protective mechanism of oral probiotic consumption in sepsis.

Automated summary

Disruption of the intestinal barrier is a cause and consequence of sepsis. Intestinal stem cell (ISC) proliferation and differentiation play a role in regenerating the intestinal epithelial cells and repairing the damaged intestinal mucosal barrier. This study demonstrates that treatment with Lactobacillus rhamnosus GG (LGG) improves the survival rate of septic mice and promotes colon epithelial cell proliferation. In vitro experiments show that LGG supernatant protects colonoids from TNF-alpha-induced damage. The LGG-induced barrier repair process is associated with increased expression of Lgr5(+) and lysozyme(+) cells, potentially mediated by the upregulation of IL-17, retinol metabolism, NF-kappa B, and the MAPK signaling pathways.