Molecular Investigation of Theileria and Babesia Species in Domestic Mammals from Sardinia, Italy

Simple Summary Babesia and Theileria are tick-borne parasites in the Phylum Apicomplexa with considerable impact on animal and human health globally. In this study, 152 blood samples collected from Sardinian mammals were analyzed by PCR and sequencing targeting the 18S rRNA gene specific for the Babesia/Theileria species. Babesia bigemina and Theileria orientalis/sergenti/buffeli were detected in the cattle, while the DNA of Theileria equi was found in the horses. In addition, the presence of Theileria annulata was demonstrated for the first time in cattle from Sardinia. These results improve the epidemiological knowledge of piroplasm infections in cattle and horses from the study area and highlight that additional research is warranted to determine the prevalence of Babesia and Theileria infections in Sardinia. Piroplasmoses are tick-borne diseases caused by hemoprotozoan parasites of veterinary and public health significance. This study focuses on the molecular identification and characterization of species belonging to the Theileria/Babesia genera in 152 blood samples, collected from 80 horses and 72 cattle from several farms in Sardinia, by targeting the 18S rRNA gene. The PCR results highlighted that 72% of the samples were positive for Theileria/Babesia spp., with a rate of infection of 68% and 75% for the horses and cattle, respectively. Sequencing and the BLASTn analysis showed that the 18S rRNA generated in this study has 99-100% homology with the B. bigemina, T. orientalis/sergenti/buffeli, T. equi and T. annulata strains isolated from different hosts worldwide. These findings improve the knowledge on Babesia and Theileria infections in domestic mammals and confirm the significant prevalence of piroplasmosis among subclinical and carrier animals throughout the island. Furthermore, the presence of T. annulata, reported for the first time in the study area, expands the repertoire of pathogens already detected in Sardinia. Our results gather updates on the diversity and distribution of piroplasms in Sardinia and suggest the need to develop procedures to improve animal and public health safety.

Automated summary

In this study, Babesia and Theileria infections were investigated in 152 blood samples from Sardinian mammals using PCR and sequencing. It was found that Babesia bigemina and Theileria orientalis/sergenti/buffeli were present in cattle, while Theileria equi was found in horses. Additionally, Theileria annulata was detected in cattle in Sardinia for the first time. These findings contribute to the epidemiological understanding of piroplasm infections and highlight the need for further research to determine the prevalence of Babesia and Theileria infections in Sardinia.