Optimization of the culture of Chlorella sorokiniana PA.91 by RSM: effect of temperature, light intensity, and MgAC-NPs

The unique physicochemical properties of magnesium amino clay nanoparticles (MgAC-NPs) tends to be beneficial in the application as a co-additive in treating microalgae. Also, MgAC-NPs can create oxidative stress in the environment, concurrently elective control bacteria in mixotrophic culture, and stimulate CO2 biofixation. The condition of the cultivation of newly isolated strains, Chlorella sorokiniana PA.91, was optimized for the first time for MgAC-NPs at various temperatures and light intensities in the culture medium of municipal wastewater (MWW) by central composite design in the response surface methodology (RSM-CCD). This study examined synthesized MgAC-NP with their FE-SEM, EDX, XRD, and FT-IR characteristics. The synthesized MgAC-NPs were naturally stable, cubic shaped, and within the size range of 30-60 nm. The optimization results show that at culture conditions of 20 degrees C, 37 mu mol m(-2) s(-1), and 0.05 g L-1, microalga MgAC-NPs have the best growth productivity and biomass performance. Maximum dry biomass weight (55.41%), specific growth rate (30.26%), chlorophyll (81.26%), and carotenoids (35.71%) were achieved under the optimized condition. Experimental results displayed that C.S. PA.91 has a high capacity for lipid extraction (1.36 g L-1) and significant lipid efficiency (45.1%). Also, in 0.2 and 0.05 g L-1 of the MgAC-NPs, COD removal efficiency 91.1% and 81.34% from C.S. PA.91 showed, respectively. These results showed the potential of C.S. PA.91-MgAC-NPs for nutrient removal in wastewater treatment plants and their quality as sources of biodiesel.

Automated summary

The physicochemical properties of MgAC-NPs make them beneficial in treating microalgae and have the potential for nutrient removal in wastewater treatment plants. The optimized conditions for cultivating Chlorella sorokiniana PA.91 with MgAC-NPs were found to be 20 degrees C, 37 μmol m(-2) s(-1), and 0.05 g L-1, resulting in the best growth productivity and biomass performance.