Structural and mechanical properties of folded protein hydrogels with embedded microbubbles

Globular folded proteins are powerful building blocks to create biomaterials with mechanical robustness and inherent biological functionality. Here we explore their potential as advanced drug delivery scaffolds, by embedding microbubbles (MBs) within a photo-activated, chemically cross-linked bovine serum albumin (BSA) protein network. Using a combination of circular dichroism (CD), rheology, small angle neutron scattering (SANS) and microscopy we determine the nanoscale and mesoscale structure and mechanics of this novel multi-composite system. Optical and confocal microscopy confirms the presence of MBs within the protein hydrogel, their reduced diffusion and their effective rupture using ultrasound, a requirement for burst drug release. CD confirms that the inclusion of MBs does not impact the proportion of folded proteins within the cross-linked protein network. Rheological characterisation demonstrates that the mechanics of the BSA hydrogels is reduced in the presence of MBs. Furthermore, SANS reveals that embedding MBs in the protein hydrogel network results in a smaller number of clusters that are larger in size (similar to 16.6% reduction in number of clusters, 17.4% increase in cluster size). Taken together, we show that MBs can be successfully embedded within a folded protein network and ruptured upon application of ultrasound. The fundamental insight into the impact of embedded MBs in protein scaffolds at the nanoscale and mesoscale is important in the development of future platforms for targeted and controlled drug delivery applications.

Automated summary

Globular folded proteins have been explored as powerful building blocks for developing biomaterials with mechanical strength and biological functionality. In this study, microbubbles were embedded in a cross-linked bovine serum albumin (BSA) protein network for advanced drug delivery scaffolds. Various characterization techniques were used to determine the structure and mechanics of this multi-composite system. The results demonstrate successful embedding of microbubbles within the folded protein network, which can be ruptured using ultrasound for burst drug release. This fundamental insight into the impact of embedded microbubbles in protein scaffolds is crucial for the development of targeted and controlled drug delivery platforms.