A sensitive dual-signal electrochemiluminescence immunosensor based on Ru(bpy)32+@HKUST-1 and Ce2Sn2O7 for detecting the heart failure biomarker NT-proBNP

A sensitive dual-signal electrochemiluminescence (ECL) immunosensor was proposed based on Ru(bpy)(3)(2+)@HKUST-1/TPA and Ce2Sn2O7/K2S2O8 probes for detecting the NT-proBNP biomarker of heart failure. HKUST-1 with a high specific surface area facilitates the loading of more Ru(bpy)(3)(2+), effectively improving the anodic signal intensity, while the emerging Ce2Sn2O7 emitter displays a potential-matching cathodic emission with moderate intensity. Two ECL probes were characterized with field emission scanning electron microscopy, X-ray diffraction, XPS, FT-IR spectroscopy and UV-Vis diffuse reflectance spectroscopy. This dual-signal immunosensor has a wide linear range (5 x 10(-4)-1 x 10(4) ng mL(-1)) and a low quantitative detection limit, simultaneously showing high sensitivity, stability and reproducibility, as well as the detection capability of actual serum samples. This dual signal-calibrated immunoassay platform not only reduces the false positive rate of detection results but also provides a promising method for the early diagnosis of heart failure.

Automated summary

A sensitive dual-signal electrochemiluminescence (ECL) immunosensor was developed using Ru(bpy)(3)(2+)@HKUST-1/TPA and Ce2Sn2O7/K2S2O8 probes for detecting the NT-proBNP biomarker of heart failure. The HKUST-1 facilitated the loading of more Ru(bpy)(3)(2+), improving the signal intensity, while the Ce2Sn2O7 emitter showed a potential-matching cathodic emission. The immunosensor demonstrated a wide linear range, low detection limit, high sensitivity, stability, reproducibility, and the ability to detect actual serum samples.