Efficacious use of potential biosurfactant producing plant growth promoting rhizobacteria to combat petrol toxicity in Zea mays L. plants

Soil pollution caused by petroleum hydrocarbons is a serious threat for human life, as it affects the groundwater, cause economical losses after decreasing the agricultural production, and create other ecological issues. Here, we are reporting the isolation and screening of rhizosphere bacteria possessing biosurfactant producing potential and capable of enhancing plant growth under petrol stress as well as possessing. Efficient biosurfactant producers having plant growth promoting traits were characterized morphologically, physiologically, and phylogenetically. These selected isolates were identified as Bacillus albus S2i, Paraclostridium benzoelyticum Pb4, and Proteus mirabilis Th1 based on 16S rRNA sequence analysis. These bacteria possessed plant growth promoting attributes as well as exhibited positive activity toward the assays based on hydrophobicity, lipase activity, surface activity, and hydrocarbon degradation that indicated the production of biosurfactants. Fourier transform infrared spectroscopy of crude biosurfactants extracted from bacterial strains revealed that the biosurfactants from Pb4 and Th1 might belong to glycolipid or glycolipopeptide class whereas the biosurfactants from S2i could be from phospholipid class. Scanning electron micrographs exhibited group of exopolymer matrices interconnecting the cells forming a complex network of mass, while energy dispersive X-ray analysis has shown elemental composition of biosurfactants with dominance of nitrogen, carbon, oxygen, and phosphorous. Further, these strains were then used to ascertain their effect on the growth and biochemical parameters including stress metabolites and antioxidant enzymology of Zea mays L. plants grown under petrol (gasoline) stress. Significant increments in all studied parameters were observed in comparison with control treatments that might be due to petrol degradation by bacteria and also by secreting growth stimulating substances released by these bacteria in soil ecosystem. To the best of our knowledge, this is the first report in which Pb4 and Th1 were studied as surfactant producing PGPR and further their role as biofertilizer for the significant improvement in phytochemical constituents of maize plants grown under petrol stress was assessed.

Automated summary

Soil pollution caused by petroleum hydrocarbons is a serious threat to human life, affecting groundwater, agricultural production, and ecological issues. In this study, we isolated and screened rhizosphere bacteria that produce biosurfactants and enhance plant growth under petrol stress. These bacteria exhibited plant growth promoting traits and positive activity towards hydrophobicity, lipase activity, surface activity, and hydrocarbon degradation assays. Crude biosurfactants extracted from these bacteria showed potential belonging to different classes. Scanning electron micrographs revealed complex networks of exopolymer matrices, while energy dispersive X-ray analysis confirmed the elemental composition of biosurfactants. Furthermore, these bacteria significantly improved the growth and biochemical parameters of Zea mays L. plants grown under petrol stress, indicating their role as biofertilizers.