期刊
BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
卷 16, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13068-023-02291-6
关键词
Inhibitor tolerance; Biofuels; Clostridium thermocellum; Spermidine; Butanol dehydrogenase
Co-expression with an NADPH-dependent heat-stable butanol dehydrogenase from Thermoanaerobacter pseudethanolicus enhances tolerance of Clostridium thermocellum to furans and acetic acid and increases thermotolerance at 65 degrees C.
BackgroundSensitivity to inhibitors derived from the pretreatment of plant biomass is a barrier to the consolidated bioprocessing of these complex substrates to fuels and chemicals by microbes. Spermidine is a low molecular weight aliphatic nitrogen compound ubiquitous in microorganisms, plants, and animals and is often associated with tolerance to stress. We recently showed that overexpression of the endogenous spermidine synthase enhanced tolerance of the Gram-positive bacterium, Clostridium thermocellum to the furan derivatives furfural and HMF.ResultsHere we show that co-expression with an NADPH-dependent heat-stable butanol dehydrogenase from Thermoanaerobacter pseudethanolicus further enhanced tolerance to furans and acetic acid and most strikingly resulted in an increase in thermotolerance at 65 degrees C.ConclusionsTolerance to fermentation inhibitors will facilitate the use of plant biomass substrates by thermophiles in general and this organism in particular. The ability to grow C. thermocellum at 65 degrees C has profound implications for metabolic engineering.
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