Optimizing ozone dose and contact time for removal of antibiotic-resistant P. aeruginosa, A. baumannii, E. coli, and associated resistant genes in effluent of an activated sludge process in a municipal WWTP

This study aimed to investigate the impact of ozonation on inactivation of antibiotic-resistant bacteria (ARB) including E. coli, P. aeruginosa, and A. baumannii, as well as on removal of 16S-rRNA gene and their associated antibiotic-resistant genes (ARGs) indigenously present in effluent of municipal wastewater treatment plant. The Chick-Watson model was used to describe bacterial inactivation rates at specific ozone doses. Maximum reduction of total cultivable A. baumannii, E. coli, and P. aeruginosa were found to be 7.6, 7.1, and 4.7 log, respectively, with the highest ozone dose of 0.48 gO(3)/gCOD at 12 min contact time. According to the study results, complete inactivation of ARB and bacterial regrowth was not observed after 72 h incubation. The culture methods overestimated the performance of disinfection processes and propidium monoazide combined with qPCR, and showed the presence of viable but non-culturable bacteria after ozonation. ARGs were more persistent to ozone than ARB. The results of this study highlighted the significance of specific ozone dose and contact time in ozonation process considering the bacterial species and associated ARGs as well as the wastewater physicochemical characteristics, in order to help diminish the entrance of the biological microcontaminants into the environment.

Automated summary

This study aimed to investigate the impact of ozonation on inactivation of antibiotic-resistant bacteria (ARB) and removal of associated antibiotic-resistant genes (ARGs) in effluent of a municipal wastewater treatment plant. The results showed that specific ozone doses followed the Chick-Watson model for bacterial inactivation rates. The highest reductions in A. baumannii, E. coli, and P. aeruginosa were achieved with a ozone dose of 0.48 gO(3)/gCOD at 12 minutes contact time. Complete inactivation of ARB and bacterial regrowth were not observed after 72 hours incubation. ARGs were found to be more persistent to ozone than ARB.