Chronic toxicity of broflanilide in Daphnia magna: changes in molting, behavior, and gene expression

Broflanilide is a novel pesticide used in agriculture that binds to unique receptors on pests; however, the widespread use of broflanilide has led to toxicity in Daphnia magna. At present, little information on the potential threats broflanilide imposes on D. magna is available. Therefore, the present study examined the chronic toxicity of broflanilide in D. magna by comparing changes in molting, neurotransmitter function, and behavior. The results showed that broflanilide caused chronic toxicity in D. magna at a concentration of 8.45 mu g/L, and growth, development, reproduction, and the development of offspring were affected. In addition, broflanilide affected the molting of D. magna by significantly inhibiting the expression of chitinase, ecdysteroid, and related genes. Broflanilide also affected the expression of gamma-glutamic acid, glutamine, gamma-aminobutyric acid, 5-hydroxytryptamine, 5-hydroxytryptophan, dopa, and dopamine. Furthermore, the swimming distance and speed of D. magna were reduced. Taken together, the results demonstrate the chronic toxicity and exposure risk of broflanilide in D. magna.

Automated summary

This study investigated the chronic toxicity of the novel pesticide broflanilide on Daphnia magna. The results showed that broflanilide affected the growth, development, reproduction, and offspring development of D. magna. It inhibited the expression of chitinase, ecdysteroid, and related genes, affecting molting. Broflanilide also affected the neurotransmitter function and behavior of D. magna, reducing swimming distance and speed.