期刊
VETERINARY RESEARCH
卷 54, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13567-023-01161-1
关键词
Porcine edema disease; Shiga toxin 2e; vaccine; Stx2e neutralizing antibody; Stx2e B subunit; cartilage oligomeric matrix protein
A recombinant protein containing Stx2eB-COMP was constructed as a vaccine, which showed significant efficacy in preventing ED by inducing neutralizing antibodies against Stx2e. The vaccine group exhibited higher neutralizing antibody titers, lower mortality and clinical scores compared to the control group. These findings suggest that the pentameric B subunit vaccine is a promising tool for preventing ED in pigs.
Porcine edema disease (ED) is an enterotoxaemia that frequently occurs in 4-12 week-old piglets and results in high mortality. ED is caused by Shiga toxin 2e (Stx2e), produced by host-adapted Shiga toxin-producing Escherichia coli (STEC) strains. We constructed a recombinant protein in which the B subunit of Stx2e (Stx2eB) was linked to Cartilage Oligomeric Matrix Protein (COMP)'s pentameric domain to enhance antigenicity to induce neutralizing antibodies against Stx2e. We evaluated the efficacy of this antigen as a vaccine on the farm where ED had occurred. The suckling piglets were divided into two groups. The pigs in the vaccinated group were intramuscularly immunized with the vaccine containing 30 mu g/head of Stx2eB-COMP at 1 and 4 weeks of age. The control pigs were injected with saline instead of the vaccine. The neutralizing antibody titer to Stx2e, mortality, clinical score, and body weight was evaluated up to 11 weeks after the first vaccination. In the vaccinated group, the Stx2e neutralizing antibody was detected 3 weeks after the first vaccination, its titer increased during the following weeks. The antibody was not detected in the control group during the test period. The STEC gene was detected in both groups during the test period, but a typical ED was observed only in control pigs; the mortality and clinical score were significantly lower in the vaccinated group than in the control group. These data indicate that the pentameric B subunit vaccine is effective for preventing ED and offers a promising tool for pig health control.
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