Species differences in stoichiometric homeostasis affect grassland community stability under N and P addition

Unbalanced N and P input has substantially altered the relative importance of N and P limitation in grassland ecosystems, which resulted in profound impacts on species nutrient cycling, community structure, and ecosystem stability. However, the underlying species-specific nutrient use strategy and stoichiometric homeostasis in driving community structure and stability changes remain unclear. A split-plot N and P addition experiment (main-plot: 0, 25, 50, and 100 kgN hm(-2) a(-1); subplot: 0, 20, 40, and 80 kgP(2)O(5) hm(-2) a(-1)) was conducted during 2017-2019 in two typical grasslands (perennial grass and perennial forb) communities in the Loess Plateau. The stoichiometric homeostasis of 10 main component species, species dominance, stability changes, and their contribution to community stability were investigated. Perennial legume and perennial clonal species tend to perform higher stoichiometric homeostasis than non-clonal and annual forb. Large shifts in species with high homeostasis vs. low homeostasis caused by N and P addition showed consistently profound impacts on community homeostasis and stability in both communities. In both two communities, species dominance performed significantly positive relationships with homeostasis under no N and P addition. P alone or combined with 25 kgN hm(-2) a(-1) addition strengthened species dominance-homeostasis relationship and increased community homeostasis due to increased perennial legumes. Under 50 and 100 kgN hm(-2) a(-1) combined with P addition, species dominance-homeostasis relationships were weakened, and community homeostasis decreased significantly in both communities, which was due to that increased annual and non-clonal forb suppressed perennial legume and clonal species. Our results demonstrated that trait-based classifications of species-level homeostasis offer a reliable tool in predicting species performance and community stability under N and P addition, and conserving species with high homeostasis is important to enhance semiarid grassland ecosystem function stability on the Loess Plateau.

Automated summary

Unbalanced N and P input has significant impacts on species nutrient cycling, community structure, and ecosystem stability in grassland ecosystems. A stoichiometric homeostasis classification based on traits provides a reliable tool to predict species performance and community stability.