期刊
REPRODUCTION AND FERTILITY
卷 4, 期 1, 页码 -出版社
BIOSCIENTIFICA LTD
DOI: 10.1530/RAF-22-0106
关键词
sperm; cryostorage; spermatogenesis; male infertility; ICSI
Sperm cryopreservation is commonly used for short-term sperm storage in men with severely impaired spermatogenesis, especially before fertility treatment. This review discusses various aspects of freezing sperm, including methods overview, appropriate use of cryoprotectants, practical considerations, as well as oxidative stress and mechanisms of cell cryodamage.
Sperm cryopreservation for men with severely impaired spermatogenesis is one of the commonest reasons for shortterm sperm storage, usually in advance of fertility treatment. Cryopreservation is generally very effective, although not all spermatozoa survive the process of freezing and thawing. This review considers various aspects of freezing sperm, including an overview of methods, appropriate use of cryoprotectants and practical considerations, as well as oxidative stress and mechanisms of cell cryodamage.
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