Tetracycline resistance potential of heterotrophic bacteria isolated from freshwater fin-fish aquaculture system

Aims: This study investigated the tetracycline resistance potential of heterotrophic bacteria isolated from twenty-four freshwater fin-fish culture ponds in Andhra Pradesh, India. Methods and results A total of 261 tetracycline resistant bacteria (tet(R)) were recovered from pond water, pond sediment, fish gills, fish intestine, and fish feed. Bacteria with high tetracycline resistance (tet(HR)) (n = 30) that were resistant to tetracycline concentrations above 128 mu g mL(-1) were predominantly Lactococcus garvieae followed by Enterobacter spp., Lactococcus lactis, Enterobacter hormaechei, Staphylococcus arlettae, Streptococcus lutetiensis, Staphylococcus spp., Brevundimonas faecalis, Exiguobacterium profundum, Lysinibacillus spp., Stutzerimonas stutzeri, Enterobacter cloacae, and Lactococcus taiwanensis. Resistance to 1024 mu g mL(-1) of tetracycline was observed in L. garvieae, S. arlettae, Enterobacter spp., B. faecalis. Tet(A) (67%) was the predominant resistance gene in tet(HR) followed by tet(L), tet(S), tet(K), and tet(M). At similar concentrations of exposure, tetracycline procured at the farm level (69.5% potency) exhibited lower inhibition against tet(HR) bacteria compared to pure tetracycline (99% potency). The tet(HR) bacteria showed higher cross-resistance to furazolidone (100%) followed by co-trimoxazole (47.5%) and enrofloxacin (11%). Conclusions The maximum threshold of tetracycline resistance at 1024 mu g mL(-1) was observed in S. arlettae, Enterobacter spp., B. faecalis, and L. garvieae and tet(A) was the major determinant found in this study.

Automated summary

This study investigated the tetracycline resistance potential of heterotrophic bacteria isolated from freshwater fin-fish culture ponds in Andhra Pradesh, India. The results showed that Lactococcus garvieae was the predominant bacteria with high tetracycline resistance, followed by Enterobacter spp., Lactococcus lactis, and Staphylococcus arlettae. The tet(A) gene was the major determinant of tetracycline resistance. The tet(HR) bacteria showed cross-resistance to furazolidone, co-trimoxazole, and enrofloxacin.