A 3D printable synthetic hydrogel as an immobilization matrix for continuous synthesis with fungal peroxygenases

Enzyme immobilization is the key to an intensified bioprocess that allows recycling of the heterogenized enzyme and/or continuous biocatalytic production. In this communication, we present a case study for enzyme immobilization in a novel, 3D printable synthetic hydrogel and its use in continuous oxidation reactions. Immobilization resulted in an average immobilization yield of 6.1% and continuous synthesis was run for 24 hours with a space-time yield of 3.1 x 10(-2) g L-1 h(-1).

Automated summary

This study demonstrates the immobilization of enzymes in a novel 3D printable synthetic hydrogel and its application in continuous oxidation reactions. The average immobilization yield was 6.1%, and the continuous synthesis reaction lasted for 24 hours with a space-time yield of 3.1 x 10(-2) g L-1 h(-1).