期刊
SMALL SCIENCE
卷 3, 期 5, 页码 -出版社
WILEY
DOI: 10.1002/smsc.202300002
关键词
bacteria; material interfaces; carbon nanotubes; fluorescence microscopy; mechanobactericidal strategies; Min oscillations; nanofabricated topographies; real-time imaging
Mechanobactericidal nanomaterials are promising antimicrobial strategies that rely on mechanical interactions with bacteria and overcome antibiotic resistance. However, the detailed mechanisms behind the killing effects are not well understood. This study investigates the real-time response of bacteria to weak mechanical interactions with nanostructured topographies and nanodarts, shedding light on the complex mechanisms of mechanically induced bacterial death.
Mechanobactericidal nanomaterials, such as low-dimensional nanoparticles in suspension or high-aspect-ratio nanofabricated topographies, rely on their mechanical or physical interactions with bacteria and are promising antimicrobial strategies that overcome bacterial resistance to classical antibiotics. However, the underlying killing mechanisms are poorly understood, given the challenges associated with the real-time characterization of the mechanical interaction in a biologically relevant environment. Indeed, different death mechanisms have been proposed depending on the magnitude of the interaction forces. Herein, the real-time and single-cell response of bacteria to weak mechanical interactions with nanostructured topographies and nanodarts, exemplified by flowing single-walled carbon nanotubes, is investigated. To that end, an advanced reporting strategy is used to follow sublethal physiological effects on bacteria upon contact with these materials. With this method, it is estimated that the contact time at which the initial stages of bacterial death occur is in the order of a few tens of minutes. This information contributes to a full understanding of the complex mechanisms of mechanically induced bacterial death.
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