Single-Cell Isolation Microfluidic Chip Based on Thermal Bubble Micropump Technology

The isolation of single cells is essential for the development of single cell analysis methods, such as single-cell sequencing, monoclonal antibodies, and drug development. Traditional single-cell isolation techniques include flow cytometry (FACS), laser capture microdissection (LCM), micromanipulation, etc., but their operations are complex and have low throughput. Here, we present a microfluidic chip that can isolate individual cells from cell suspension and release them onto a well plate. It uses thermal bubble micropump technology to drive the fluid flow, and single-cell isolation is achieved by matching the flow resistance of the flow channel. Therefore, injection pumps and peristaltic pumps are not required for cell loading. Because of its small size, we can integrate hundreds of single-cell functional modules, which makes high-throughput single-cell isolation possible. For polystyrene beads, the capture rate of the single bead is close to 100%. Finally, the method has been applied to cells, and the capture rate of the single cell is also about 75%. This is a promising method for single-cell isolation.

Automated summary

The isolation of single cells is crucial for the advancement of single cell analysis methods. Traditional techniques have limitations in terms of complexity and throughput. A microfluidic chip has been developed using thermal bubble micropump technology, allowing for efficient single-cell isolation without the need for injection pumps or peristaltic pumps. The method has shown high capture rates for both polystyrene beads and cells, making it a promising approach for single-cell isolation.