Designing protein nano-construct in ionic liquid: a boost in efficacy of cytochrome C under stresses

Herein, we present a simple approach to fabricate protein nanoconstructs by complexing cytochrome C (Cyt C) with silk nanofibrils (SNF) and choline dihydrogen phosphate ionic liquid (IL). The peroxidase activity of the IL modified Cyt C nanoconstruct (Cyt C + SNF + IL) increased significantly (2.5 to 10-fold) over unmodified Cyt C and showed enhanced catalytic activity and stability under harsh conditions, proving its potential as a suitable protein packaging strategy.

Automated summary

In this study, a simple approach to fabricate protein nanoconstructs using cytochrome C (Cyt C), silk nanofibrils (SNF), and choline dihydrogen phosphate ionic liquid (IL) was presented. The modified IL-Cyt C nanoconstruct (Cyt C + SNF + IL) showed significantly increased peroxidase activity (2.5 to 10-fold) compared to unmodified Cyt C and exhibited enhanced catalytic activity and stability under harsh conditions, suggesting its potential as a suitable protein packaging strategy.