期刊
CIENCIA RURAL
卷 53, 期 11, 页码 -出版社
UNIV FEDERAL SANTA MARIA
DOI: 10.1590/0103-8478cr20210883
关键词
tomato chlorosis virus; tomato severe rugose virus; whitefly
类别
In recent decades, the transmission of viruses by whiteflies has become a problem in tomato fields, posing a threat to potato crops. This study conducted a survey of begomoviruses and criniviruses in tomato and potato crops in the state of Parana, Brazil, from 2015 to 2018. PCR and RT-PCR were used to detect begomoviruses and criniviruses, respectively, using total DNA and RNA extracted from leaves. Results showed a low incidence (6.5%) of crinivirus infecting tomato crops in Parana State, with one amplified fragment sharing 99.71% identity with tomato chlorotic virus (ToCV).
In the last decades, the high incidence of viruses transmitted by whiteflies has become a problem in the tomato fields, threatening, more recently, the potato crops. The present study carried out a survey of begomoviruses and criniviruses in tomato and potato crops, from 2015 to 2018, in the municipalities of Araucaria, Campo do Tenente, Campo Largo, Contenda, Lapa, Faxinal, Morretes, Reserva, Castro, Palmeira and Sao Mateus do Sul, in Parana State, Brazil. Total DNA and RNA from leaves were extracted and used as templates to detect, respectively, begomoviruses by PCR and criniviruses by RT-PCR. Out of 215 tomato samples, 14 from Faxinal were infected by crinivirus. The other tomato samples and 243 potato samples showed negative results for begomovirus and crinivirus. Results indicated a low incidence (6.5%) of crinivirus infecting tomato crops in Parana State, and the nucleotide sequence of one amplified fragment shared 99.71% identity with tomato chlorotic virus (ToCV).
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