Seven new triterpenoid saponins from Astragalus membranaceus var. mongholicus and the inhibition of high-glucose induced SV40 MES 13 cells

A phytochemical study of the ethanol extract of Astragalus membranaceus (Fisch) Bge. var. mongholicus (Bge) Hsiao resulted in the isolation of seven new triterpenoid saponins, astralanosaponins F-L, together with fifteen known compounds (8-22). Structure analysis of these extracted compounds was performed using 1D-NMR and 2D-NMR spectroscopy, and their data were compared with those of similar compounds previously reported in the literature. The absolute configuration of sugar was determined using GC-MS. The cell viability of SV40 MES 13 cells induced by high glucose was tested using the CCK-8 assay. Compound 19 showed obvious inhibitory effects of the high-glucose induced SV40 MES 13 cells with IC50 values of 12.2 + 1.29 mM, while compounds 5 and 9 showed moderate inhibitory effects with IC50 less than 30 mM. Their possible structure-activity relationships were preliminarily summarized.

Automated summary

In this study, a phytochemical investigation of Astragalus membranaceus (Fisch) Bge. var. mongholicus (Bge) Hsiao ethanol extract led to the identification of seven new triterpenoid saponins, astralanosaponins F-L, along with fifteen known compounds. The structures of these compounds were determined through 1D-NMR and 2D-NMR spectroscopy and compared with similar compounds reported previously. The absolute configuration of sugar was determined using GC-MS. The inhibitory effects of these compounds on high glucose-induced SV40 MES 13 cells were evaluated using the CCK-8 assay, and compound 19 showed significant inhibition with an IC50 value of 12.2 + 1.29 mM. Compounds 5 and 9 also displayed moderate inhibitory effects with IC50 values below 30 mM. The possible structure-activity relationships were preliminarily summarized.