Microfluidic methods used in exosome isolation

Article Engineering, Biomedical

Isolation of exosome from the culture medium of Nasopharyngeal cancer (NPC) C666-1 cells using inertial based Microfluidic channel

Boon Yew Teoh et al.

Summary: This study developed an inertial microfluidic channel to efficiently separate exosomes from cell culture medium, showing its potential advantages compared to conventional extraction kits.

BIOMEDICAL MICRODEVICES (2022)

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Review Biotechnology & Applied Microbiology

The Yin and Yang of exosome isolation methods: conventional practice, microfluidics, and commercial kits

Saeedreza Zeibi Shirejini et al.

Summary: This article summarizes the conventional and microfluidic-based methods for exosome isolation, highlighting their strengths and limitations. Microfluidic devices, in particular, offer a promising approach to efficiently isolate exosomes, providing unprecedented tools for molecular biology and clinical applications in exosome research.

BIOTECHNOLOGY ADVANCES (2022)

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Article Pharmacology & Pharmacy

Development of a New Drug Delivery System from HELA-Derived Exosomes and the Effect of Docetaxel-Loaded Exosomes on Mitochondrial Apoptosis

Melike Cenik et al.

Summary: In this study, a new drug delivery system was developed by loading docetaxel into exosomes derived from HELA cells, resulting in induction of mitochondrial apoptosis in cervical cancer cells with low toxicity.

JOURNAL OF PHARMACEUTICAL INNOVATION (2022)

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Review Chemistry, Multidisciplinary

Exosome Processing and Characterization Approaches for Research and Technology Development

James J. Lai et al.

Summary: Exosomes, as extracellular vesicles, have potential as disease biomarkers and therapeutic agents. However, manufacturing high-quality exosomes is challenging due to the lack of standardized practices and reference materials. This review compares exosome processing and characterization methods, recommends exosome workflows, and provides an introduction to exosomes, including their properties and roles in biological processes and disease progression. It also summarizes ongoing clinical trials.

ADVANCED SCIENCE (2022)

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Review Cell Biology

The Role of Extracellular Vesicles in Systemic Lupus Erythematosus

Chenghui Zheng et al.

Summary: Extracellular vesicles (EVs) are small vesicles actively secreted by most cell types, which can carry various substances and play important roles in immune modulation and the pathogenesis of autoimmune diseases. This review summarizes the recent research on EVs and discusses their applications in the pathogenesis, diagnosis, and treatment of systemic lupus erythematosus (SLE).

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY (2022)

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Article Engineering, Mechanical

A Battery-Powered Fluid Manipulation System Actuated by Mechanical Vibrations

Fatih Akkoyun et al.

Summary: This study presents the design and fabrication of a low-cost and battery-operated microfluidic pump for fluid manipulation in field and remote applications. The portable pump demonstrates high flow rates and the ability to handle high viscosity fluids.

ACTUATORS (2022)

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Review Biochemistry & Molecular Biology

Microfluidic Approaches for Affinity-Based Exosome Separation

Eike K. Theel et al.

Summary: This review paper provides a toolbox for developing new microfluidic affinity devices for exosome separation.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

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Article Chemistry, Analytical

On-Chip Organoid Formation to Study CXCR4/CXCL-12 Chemokine Microenvironment Responses for Renal Cancer Drug Testing

Adem Ozcelik et al.

Summary: In this study, simple and low-cost microfluidic devices were designed and fabricated to develop an organoid model for drug testing for renal cancers. The results showed that the standard renal cancer organoids made on a lab-on-a-chip system can be used to measure drug effects and tumor microenvironment responses.

BIOSENSORS-BASEL (2022)

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Article Multidisciplinary Sciences

Wharton jelly-derived mesenchymal stem cell exosomes induce apoptosis and suppress EMT signaling in cervical cancer cells as an effective drug carrier system of paclitaxel

Burcin Irem Abas et al.

Summary: MSC-derived exosomes loaded with paclitaxel showed a therapeutic effect in cervical cancer cells, inducing apoptosis and suppressing epithelial-mesenchymal transition proteins.

PLOS ONE (2022)

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Article Engineering, Mechanical

3D Printed Device for Separation of Cells and Particles by Tilted Bulk Acoustic Wave Actuation

Adem Ozcelik

Summary: The study demonstrates the feasibility of using 3D printing to fabricate microfluidic devices for lab-on-a-chip applications. The printed devices achieved continuous and efficient separation of target cells with high purity and cell recovery rates.

ACTUATORS (2022)

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Article Biology

Exosomes: Key tools for cancer liquid biopsy

Isabella Panfoli et al.

Summary: Exosomes, as key tools in liquid biopsy, are gaining importance in precision medicine for cancer. They play critical roles in promoting tumor metastasis, immune remodeling, and drug resistance through signal transduction, while effectively preserving biomarkers and aiding in assessing the status of donor cells.

BIOCELL (2022)

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Article Biology

MSCs-exosomes in regeneration medicine: Current evidence and future perspectives

Benshuai You et al.

Summary: MSCs-derived exosomes have shown various beneficial effects in regenerative medicine and possess many excellent properties, but there are still obstacles to overcome in terms of isolation, clinical trials, and safety evaluation.

BIOCELL (2022)

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Article Biochemical Research Methods

Exosome detection via the ultrafast-isolation system: EXODUS

Yuchao Chen et al.

Summary: The study introduces an ultrafast isolation system called EXODUS for efficient purification of exosomes from biofluids, with superior speed, purity, and yield compared to other isolation techniques.

NATURE METHODS (2021)

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Review Chemistry, Physical

Microfluidic-Based Exosome Analysis for Liquid Biopsy

Bingqian Lin et al.

Summary: Liquid biopsy based on exosomes provides valuable information for biomarker analysis, but faces challenges in exosome isolation and molecular detection. The advancement of microfluidics technology has significantly improved the selectivity and sensitivity of exosome capture and molecular detection.

SMALL METHODS (2021)

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Review Chemistry, Multidisciplinary

Towards microfluidic-based exosome isolation and detection for tumor therapy

Jie Wang et al.

Summary: Exosomes, nano-sized extracellular vesicles secreted by cells, have revolutionized various areas such as liquid biopsies, cancer biology, drug delivery, and immunotherapy. Microfluidic platforms show promise in overcoming the limitations of conventional techniques by offering high precision processing, microscale liquid handling, and integration with various functional units for efficient exosome separation and sensing.

NANO TODAY (2021)

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Review Chemistry, Analytical

Progress of Microfluidic Continuous Separation Techniques for Micro-/Nanoscale Bioparticles

Se-woon Choe et al.

Summary: Conventional membrane filtration techniques have limitations in the efficiency of separating biological particles, while microfluidic engineering allows for more efficient processing of rare biological samples in a continuous flow.

BIOSENSORS-BASEL (2021)

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Review Chemistry, Multidisciplinary

A Holistic Review of the State-of-the-Art Microfluidics for Exosome Separation: An Overview of the Current Status, Existing Obstacles, and Future Outlook

Lihua Ding et al.

Summary: Exosomes are small extracellular vesicles secreted by various cells into body fluids and hold great potential for disease diagnosis and therapy. However, isolating exosomes is challenging due to their heterogeneity and the complexity of bio-fluids. Recent advancements in microfluidic technologies offer an efficient system for exosome extraction, showcasing high speeds, portability, and low sample volumes.

SMALL (2021)

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Review Medicine, Research & Experimental

Exosomes in atherosclerosis: performers, bystanders, biomarkers, and therapeutic targets

Chen Wang et al.

Summary: Exosomes, nanosized lipid vesicles originating from the endosomal system, have been intensively studied for their roles in intercellular communication and their potential impacts on atherosclerosis. Recent research has focused on exosomal changes associated with atherosclerosis, cellular origins, potential functional cargos, and their potential as biomarkers and drug carriers for managing atherosclerosis.

THERANOSTICS (2021)

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Article Materials Science, Biomaterials

A magnetic surface-enhanced Raman scattering platform for performing successive breast cancer exosome isolation and analysis

Guohao Li et al.

Summary: Exosomes derived from tumors have shown promise as biomarkers for cancer detection and disease progression, but isolating and analyzing them remains challenging. A magnetic SERS platform demonstrated high sensitivity and specificity in distinguishing exosomes from different cancer cells, making it a potential system for efficient real-time liquid biopsy.

JOURNAL OF MATERIALS CHEMISTRY B (2021)

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Article Pathology

Acoustofluidic Salivary Exosome Isolation A Liquid Biopsy Compatible Approach for Human Papillomavirus-Associated Oropharyngeal Cancer Detection

Zeyu Wang et al.

JOURNAL OF MOLECULAR DIAGNOSTICS (2020)

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Article Chemistry, Analytical

Exosome Purification and Analysis Using a Facile Microfluidic Hydrodynamic Trapping Device

Mahnoush Tayebi et al.

ANALYTICAL CHEMISTRY (2020)

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Article Chemistry, Analytical

Acoustic Microfluidics

Peiran Zhang et al.

ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 13 (2020)

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Review Chemistry, Analytical

Applications of Converged Various Forces for Detection of Biomolecules and Novelty of Dielectrophoretic Force in the Applications

Seungjun Lee et al.

SENSORS (2020)

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Article Chemistry, Analytical

Submicron Particle Focusing and Exosome Sorting by Wavy Microchannel Structures within Viscoelastic Fluids

Yinning Zhou et al.

ANALYTICAL CHEMISTRY (2019)

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Review Nanoscience & Nanotechnology

Acoustofluidic separation of cells and particles

Mengxi Wu et al.

MICROSYSTEMS & NANOENGINEERING (2019)

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Review Chemistry, Analytical

Microfluidic strategies for label-free exosomes isolation and analysis

Wentao Su et al.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2019)

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Article Chemistry, Analytical

Electrokinetically Driven Exosome Separation and Concentration Using Dielectrophoretic-Enhanced PDMS-Based Microfluidics

Sergio Ayala-Mar et al.

ANALYTICAL CHEMISTRY (2019)

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Review Biotechnology & Applied Microbiology

Three decades of nucleic acid aptamer technologies: Lessons learned, progress and opportunities on aptamer development

Tao Wang et al.

BIOTECHNOLOGY ADVANCES (2019)

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Review Biochemical Research Methods

Recent progress of particle migration in viscoelastic fluids

Dan Yuan et al.

LAB ON A CHIP (2018)

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Review Biotechnology & Applied Microbiology

Isolation of Extracellular Vesicles: General Methodologies and Latest Trends

Maria Yu. Konoshenko et al.

BIOMED RESEARCH INTERNATIONAL (2018)

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Article Biochemical Research Methods

Integrated nanoscale deterministic lateral displacement arrays for separation of extracellular vesicles from clinically-relevant volumes of biological samples

Joshua T. Smith et al.

LAB ON A CHIP (2018)

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Article Multidisciplinary Sciences

Isolation of exosomes from whole blood by integrating acoustics and microfluidics

Mengxi Wu et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2017)

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Article Multidisciplinary Sciences

An integrated double-filtration microfluidic device for isolation, enrichment and quantification of urinary extracellular vesicles for detection of bladder cancer

Li-Guo Liang et al.

SCIENTIFIC REPORTS (2017)

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Article Materials Science, Multidisciplinary

Rapid purification of sub-micrometer particles for enhanced drug release and microvesicles isolation

Hui Min Tay et al.

NPG ASIA MATERIALS (2017)

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Article Chemistry, Multidisciplinary

Field-Free Isolation of Exosomes from Extracellular Vesicles by Microfluidic Viscoelastic Flows

Chao Liu et al.

ACS NANO (2017)

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Article Chemistry, Multidisciplinary

Rapid Isolation and Detection of Exosomes and Associated Biomarkers from Plasma

Stuart D. Ibsen et al.

ACS NANO (2017)

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Article Nanoscience & Nanotechnology

Nanoscale lateral displacement arrays for the separation of exosomes and colloids down to 20 nm

Benjamin H. Wunsch et al.

NATURE NANOTECHNOLOGY (2016)

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Article Biochemical Research Methods

Rapid inertial solution exchange for enrichment and flow cytometric detection of microvesicles

Jaideep S. Dudani et al.

BIOMICROFLUIDICS (2015)

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Article Biochemical Research Methods

Exosome isolation: a microfluidic road-map

A. Liga et al.

LAB ON A CHIP (2015)

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Article Cardiac & Cardiovascular Systems

Cardiomyocytes mediate anti-angiogenesis in type 2 diabetic rats through the exosomal transfer of miR-320 into endothelial cells

Xiaohong Wang et al.

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY (2014)

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Review Biochemical Research Methods

Deterministic lateral displacement for particle separation: a review

J. McGrath et al.

LAB ON A CHIP (2014)

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Article Biochemical Research Methods

Ciliated micropillars for the microfluidic-based isolation of nanoscale lipid vesicles

Zongxing Wang et al.

LAB ON A CHIP (2013)

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Review Physiology

Alternative methods for characterization of extracellular vesicles

Fatemeh Momen-Heravi et al.

FRONTIERS IN PHYSIOLOGY (2012)

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Article Cell Biology

Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells

Hadi Valadi et al.

NATURE CELL BIOLOGY (2007)

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Microfluidic methods used in exosome isolation

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