EFFECT OF CULTIVATION AND NATURAL RESTORATION ON SOIL MICROBIAL FUNCTIONAL STRUCTURE IN COLD-REGION WETLANDS

In order to reveal the soil cultivation and natural restoration patterns, bacterial microbial communities and functions in cold-region wetlands, the changes of soil bacterial microbial communities and functions driven by soil cultivation and natural restoration were studied by 16SrRNA Gene Fragments and their high-throughput sequencing, relying on the wetland and farmland soils at Hongxing Wetland National of Heilongjiang Province in China. The results showed that Actinobacteriota, Acidobacteriota, Proteobacteria, Chloroflexi and Firmicutes were the dominant bacteria in the tested soil samples. PCA and NMDS analysis showed that there was a significant separation between the bacterial communities in soil natural restoration treatments (CRMW and CSFW). Actinobacteria, Proteobacteria, Acidobacteria and Chloroflexi were the dominant bacterial species in the cultivation farmland (RF and SF). According to FAPROTAX algorithm and BugBase phenotype analysis, soil cultivation and natural restoration patterns can change the abundance of soil functional microorganisms. The dominant functional genes were mainly chemoheterotrophy and aerobic chemoheterotrophy bacteria. Soil cultivation and natural restoration patterns lead to significant differences between aerobic, facultatively anaerobic and stress tolerant bacteria abundances in wetland.

Automated summary

The study examined the impact of soil cultivation and natural restoration on bacterial microbial communities and functions in cold-region wetlands using 16SrRNA gene fragments and high-throughput sequencing. The dominant bacteria in the tested soil samples were Actinobacteriota, Acidobacteriota, Proteobacteria, Chloroflexi, and Firmicutes. Analysis revealed significant differences in bacterial communities between natural restoration and cultivation farmland. Functional analysis showed that soil cultivation and natural restoration patterns can alter the abundance of soil functional microorganisms, particularly chemoheterotrophy and aerobic chemoheterotrophy bacteria. These patterns also resulted in significant differences in the abundance of aerobic, facultatively anaerobic, and stress tolerant bacteria in wetland.