Three bis(anilino)-substituted NIR-AZA fluorophores were designed, synthesized, and tested for live-cell microscopy imaging in the 800-850 nm spectral range. The synthetic route allowed for the introduction of tailored peripheral substituents to guide sub-cellular localization and imaging. Successful imaging of lipid droplets, plasma membrane, and cytosolic vacuoles was achieved, and the photophysical and ICT properties of each fluorophore were examined.
Three bis(anilino)-substituted NIR-AZA fluorophores have been designed, synthesized and tested to bridge the availability gap of molecular fluorophores for live-cell microscopy imaging in the 800-850 nm spectral range. The concise synthetic route allows for the later stage introduction of three tailored peripheral substituents which guides the sub-cellular localization and imaging. Live-cell fluorescence imaging of lipid droplets, plasma membrane and cytosolic vacuoles was successfully achieved. Photophysical and internal charge transfer (ICT) properties of each fluorophore were examined through solvent studies and analyte responses.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据