All-electrical antibiotic susceptibility and resistance profiling of electrogenic Pseudomonas aeruginosa

Article Biophysics

Rapid differentiation of antibiotic-susceptible and -resistant bacteria through mediated extracellular electron transfer

Gretchen Tibbits et al.

Summary: This study proposes a rapid and direct method for classifying antibiotic susceptibility using mediated extracellular electron transfer, effectively categorizing bacteria as either resistant or susceptible.

BIOSENSORS & BIOELECTRONICS (2022)

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Review Chemistry, Multidisciplinary

Electrogenic Bacteria Promise New Opportunities for Powering, Sensing, and Synthesizing

Seokheun Choi

Summary: Considerable research has been done on electrogenic bacteria and the potential commercial opportunities they offer. These bacteria can generate enough electricity to power portable devices, while their nanomaterials can facilitate effective interfaces and biosynthetic approaches. Interfacing electronics with microbes through extracellular electron transfer can revolutionize the field of bioelectronics.

SMALL (2022)

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Review Biotechnology & Applied Microbiology

Capturing the signal of weak electricigens: a worthy endeavour

Kartik Aiyer et al.

Summary: Recently discovered non-traditional microorganisms have the ability to generate low-intensity electric currents, marking a new chapter in electromicrobiology. These microorganisms have important implications in medicine, the environment, and biotechnology. However, their study is challenging due to the low current output.

TRENDS IN BIOTECHNOLOGY (2022)

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Article Chemistry, Medicinal

Identification of Evolutionary Trajectories Associated with Antimicrobial Resistance Using Microfluidics

Seokju Seo et al.

Summary: The use of monodisperse microdroplets in experimental evolution has shown to provide better control over environmental conditions and reveal potential new evolutionary trajectories and dynamics, including large-scale chromosomal rearrangements and amplifications. Microdroplet emulsions, with their advantages in automation and condition control, offer a high-throughput method for biomarker identification and preclinical evaluation.

ACS INFECTIOUS DISEASES (2022)

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Review Microbiology

Elucidating the Mechanisms of Action of Antimicrobial Agents

M. Ashley Hudson et al.

Summary: Despite the growing antibiotic resistance crisis, the discovery and approval rate of new antimicrobials has declined rapidly over the past 75 years. Understanding the mechanism(s) of action of newly identified antibiotics is a barrier for their further development. Recent research using profiling methods, such as thermal proteome profiling in bacteria, has provided valuable insights into the mechanisms of discovered antimicrobials. This article provides an overview of the importance of target deconvolution in antimicrobial discovery, discussing both traditional approaches and the latest advances in methodologies for identifying antimicrobial targets.

MBIO (2022)

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Article Biophysics

Accelerated antibiotic susceptibility testing of pseudomonas aeruginosa by monitoring extracellular electron transfer on a 3-D paper-based cell culture platform

Zahra Rafiee et al.

Summary: In this study, a novel approach is proposed for susceptibility testing of Pseudomonas aeruginosa, providing clinically relevant guidelines for effective treatment of infections and minimizing antibiotic resistance. The method evaluates antibiotic efficacy by monitoring the bacterial extracellular electron transfer process in the presence of antibiotics, and utilizes a paper-based culturing scaffold to promote biofilm growth. This technique allows for evidence-based antimicrobial prescribing guidance for biofilm-based infections within 96 minutes.

BIOSENSORS & BIOELECTRONICS (2022)

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Review Biotechnology & Applied Microbiology

Biofilm antimicrobial susceptibility through an experimental evolutionary lens

Tom Coenye et al.

Summary: Experimental evolution studies can provide insights into the mechanisms behind reduced antimicrobial susceptibility by repeatedly exposing bacterial populations to treatment. Including biofilms in these studies is important, and this review presents an overview of why and how to study experimental evolution in biofilms. Furthermore, the review discusses what experimental evolution has taught us about tolerance and resistance in biofilms, leading to an emerging consensus view on biofilm antimicrobial susceptibility.

NPJ BIOFILMS AND MICROBIOMES (2022)

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Article Chemistry, Analytical

Biofabrication and characterization of multispecies electroactive biofilms in stratified paper-based scaffolds

Anwar Elhadad et al.

Summary: Bioelectrochemical technologies have attracted scientific interest due to their ability to facilitate efficient electron exchange between bacteria and external electrodes. This study aims to address the lack of understanding surrounding key mechanisms determining the electron-generating capabilities and syntrophic relations within microbial communities in biofilms. The researchers develop novel biofabrication and analysis platforms using paper-based 3-D systems to accurately mimic the structure, function, and physiology of multispecies biofilms.

ANALYST (2022)

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Article Biophysics

DEPIS: A combined dielectrophoresis and impedance spectroscopy platform for rapid cell viability and antimicrobial susceptibility analysis

Pragya Swami et al.

Summary: Antimicrobial resistance (AMR) is caused by inappropriate or excessive antibiotic consumption. Early diagnosis of bacterial infections can greatly curb empirical treatment and thus AMR. The authors introduce a real-time, label-free approach called DEPIS that combines dielectrophoresis for bacterial enrichment and impedance spectroscopy for cell viability analysis in under 60 minutes. This rapid cell analyses approach can aid in faster diagnosis of bacterial infections and benefit the clinical decision-making process for antibiotic treatment, addressing the critical issue of AMR.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Multidisciplinary Sciences